Protein Info for AO353_27935 in Pseudomonas fluorescens FW300-N2E3

Annotation: succinyl-CoA--3-ketoacid-CoA transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 TIGR02429: 3-oxoacid CoA-transferase, A subunit" amino acids 5 to 217 (213 residues), 245.6 bits, see alignment E=1.9e-77 PF01144: CoA_trans" amino acids 10 to 217 (208 residues), 258.4 bits, see alignment E=2.3e-81

Best Hits

Swiss-Prot: 65% identical to SCOA_BACSU: Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit A (scoA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01028, 3-oxoacid CoA-transferase subunit A [EC: 2.8.3.5] (inferred from 95% identity to pfl:PFL_2323)

MetaCyc: 62% identical to succinyl-CoA-transferase subunit A (Helicobacter pylori 26695)
3-oxoacid CoA-transferase. [EC: 2.8.3.5]

Predicted SEED Role

"Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5)" in subsystem Catechol branch of beta-ketoadipate pathway or Leucine Degradation and HMG-CoA Metabolism or Protocatechuate branch of beta-ketoadipate pathway or Serine-glyoxylate cycle (EC 2.8.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.5

Use Curated BLAST to search for 2.8.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WQI3 at UniProt or InterPro

Protein Sequence (232 amino acids)

>AO353_27935 succinyl-CoA--3-ketoacid-CoA transferase (Pseudomonas fluorescens FW300-N2E3)
MAGFDKRVSSYEEALAGLEDGMTVISGGFGLCGIPENLIAEIKRKGTRDLTVVSNNCGVD
GFGLGVLLEDRQIRKVVASYVGENALFEKQLLSGEIEVVLTPQGTLAEKMRAGGAGIPAF
FTATGVGTPVAEGKEIREFKGRPYLMEESITGDFAIVKGWKADHFGNVIYRHTAQNFNPL
AATAGKITVVEVEEIVEPGELDPTQIHTPGIYVDRVICGTFEKRIEQRTVRK