Protein Info for AO353_27815 in Pseudomonas fluorescens FW300-N2E3

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 TIGR00435: cysteine--tRNA ligase" amino acids 2 to 457 (456 residues), 597.6 bits, see alignment E=1e-183 PF01406: tRNA-synt_1e" amino acids 15 to 313 (299 residues), 467 bits, see alignment E=6.1e-144 PF09334: tRNA-synt_1g" amino acids 35 to 136 (102 residues), 21.2 bits, see alignment E=2.2e-08 amino acids 246 to 309 (64 residues), 26.1 bits, see alignment E=7.3e-10 PF00133: tRNA-synt_1" amino acids 222 to 304 (83 residues), 26.5 bits, see alignment E=5.5e-10 PF09190: DALR_2" amino acids 340 to 401 (62 residues), 72.7 bits, see alignment E=5.9e-24

Best Hits

Swiss-Prot: 96% identical to SYC_PSEPF: Cysteine--tRNA ligase (cysS) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 96% identity to pba:PSEBR_a3718)

MetaCyc: 61% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WQG1 at UniProt or InterPro

Protein Sequence (460 amino acids)

>AO353_27815 cysteine--tRNA ligase (Pseudomonas fluorescens FW300-N2E3)
VLTIYNTLTKSKEVFKPLDGNKVRMYVCGMTVYDYCHLGHGRSMVAFDLVTRWLRFSGYD
LTYVRNITDIDDKIINRARENGESFEALTERMIAAMHEDESRLNILKPDMEPRATGHIAG
MHAMIQTLIDKGYAYAPGNGDVYYRVAKFMGYGKLSRKKIEDLRIGARIEVDESKQDPLD
FVLWKAAKPGEPSWESPWGAGRPGWHIECSVMSTCCLGETFDIHGGGSDLEFPHHENEIA
QSEAATGKTYANAWMHCGMIRINGEKMSKSLNNFFTIRDVLDKYHPEVVRYLLVSSHYRS
AINYSEDNLKDAKGALERFYHALKGLPTVPAAGGEAFVERFTQVMNDDFGTPEACAVLFE
MVREINRLRESDLNAAAGLAARLKELASVLGVLQLEADDFLQAGAEGRVDAAEVEALIQA
RLTARANKDWAESDRIRDQLTAMGVVLEDGKGGTTWRLAD