Protein Info for AO353_27015 in Pseudomonas fluorescens FW300-N2E3

Annotation: 6-O-methylguanine DNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF02805: Ada_Zn_binding" amino acids 13 to 73 (61 residues), 104.2 bits, see alignment E=5.9e-34 PF00165: HTH_AraC" amino acids 103 to 131 (29 residues), 30.1 bits, see alignment (E = 8e-11) PF12833: HTH_18" amino acids 107 to 184 (78 residues), 60.6 bits, see alignment E=2.9e-20 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 271 to 350 (80 residues), 113 bits, see alignment E=2.6e-37 PF01035: DNA_binding_1" amino acids 272 to 351 (80 residues), 117.4 bits, see alignment E=4.1e-38

Best Hits

Swiss-Prot: 51% identical to ADA_ECOLI: Bifunctional transcriptional activator/DNA repair enzyme Ada (ada) from Escherichia coli (strain K12)

KEGG orthology group: K10778, AraC family transcriptional regulator, regulatory protein of adaptative response / methylated-DNA-[protein]-cysteine methyltransferase [EC: 2.1.1.63] (inferred from 82% identity to pfo:Pfl01_2938)

MetaCyc: 51% identical to DNA-binding transcriptional dual regulator / DNA repair protein Ada (Escherichia coli K-12 substr. MG1655)
2.1.1.M37 [EC: 2.1.1.M37]; Methylated-DNA--[protein]-cysteine S-methyltransferase. [EC: 2.1.1.M37, 2.1.1.63]; 2.1.1.63 [EC: 2.1.1.M37, 2.1.1.63]

Predicted SEED Role

"ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63

Use Curated BLAST to search for 2.1.1.63 or 2.1.1.M37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W0D4 at UniProt or InterPro

Protein Sequence (367 amino acids)

>AO353_27015 6-O-methylguanine DNA methyltransferase (Pseudomonas fluorescens FW300-N2E3)
MTTAKPTTEQDPRWSAVLARDPRADGQFVYAVKTTGIYCRPSSLARLPKPENVEFFDTEE
QAQAAGYRPSKRAATDQTHLAVQHATLVAAACRQIEAAETLPSLNELAETARMSAFHFHR
VFKAVTGLTPKGYATAQRSRRVRERLEVAGSVTDALYEAGFNSNSRFYESADHLLGMKPS
DYRAAGQNTDIRFAVGQCSLGAILVAQSQRGVCAILLGDDPHALVCDLQDRFRQANLIGA
DHDFEQLIAKVVGFIEAPALGLDLPLDVRGTAFQERVWMALRDIPAGSTASYAEIAQRIG
APKAFRAVAQACGANNLAVAIPCHRVVRSDGDLSGYRWGVERKRQLLARETKSQKMPIYT
ATDSGPV