Protein Info for AO353_26885 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): phosphogluconate 2-dehydrogenase KguD (EC 1.1.1.43)
Rationale: Specific phenotype: utilization of D-Gluconic Acid and of glucose with alternate N sources. See PMC1951859
Original annotation: bifunctional glyoxylate/hydroxypyruvate reductase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 PF00389: 2-Hacid_dh" amino acids 5 to 319 (315 residues), 102.6 bits, see alignment E=1.4e-33 PF02826: 2-Hacid_dh_C" amino acids 108 to 287 (180 residues), 209.2 bits, see alignment E=3.1e-66

Best Hits

Swiss-Prot: 61% identical to GHRB_ESCF3: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 86% identity to pfo:Pfl01_2904)

MetaCyc: 85% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.215

Use Curated BLAST to search for 1.1.1.215 or 1.1.1.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WP67 at UniProt or InterPro

Protein Sequence (328 amino acids)

>AO353_26885 phosphogluconate 2-dehydrogenase KguD (EC 1.1.1.43) (Pseudomonas fluorescens FW300-N2E3)
MKKQVVLYKKLSAPLMARLHEQAEVTLIDSLDAEGLKRLREALPSAHGLLGASLKLDAEL
LDLAPRLEAIASVSVGVDNYDIDYLNRRQILLSNTPDVLTETTADTGFALILATARRVVE
LANLVRAGQWNRNIGPAHFGTDVHGKTLGIIGMGRIGEALAQRGHFGFGMPILYHSHSVK
PAVEQRFNAQYRSLPALLQQADFICLTLPLTAETEGLIGAEQFALMRPESIFINISRGKV
VDETALIEALRQGQIRAAGLDVFEREPLAYDSPLLQLNNVVATPHIGSATHETREAMARC
AVDNLLAALAGERPANLVNGQVWETQNL