Protein Info for AO353_26685 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): cobalt efflux pump
Rationale: Strong phenotypes on citrate (as a carbon source) but also on cobalt stress. The citrate phenotype may be due to iron deprivation (via chelation by the citrate) leading to heavy metal stress. The cobalt phenotype is more conserved. It is possible that nickel is also a substrate of this efflux pump, as a slight defect in nickel stress was observed, and this protein is 49% identical to the cobalt and nickel exporter dmeF (Atu0891) of Agrobacterium tumefaciens (PMID:27235438). AO353_26685 is annotated as performing zinc or cadium efflux as well (czcD), but unfortunately, we do not have data for those heavy metal stresses. However a role in zinc stress seems doubtful, as a close homolog in strain GW456-L13 is not important for resisting zinc.
Original annotation: cation transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 transmembrane" amino acids 29 to 49 (21 residues), see Phobius details amino acids 59 to 78 (20 residues), see Phobius details amino acids 97 to 117 (21 residues), see Phobius details amino acids 128 to 151 (24 residues), see Phobius details amino acids 182 to 202 (21 residues), see Phobius details amino acids 208 to 229 (22 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 25 to 308 (284 residues), 218.2 bits, see alignment E=7e-69 PF01545: Cation_efflux" amino acids 32 to 239 (208 residues), 120.9 bits, see alignment E=3.1e-39

Best Hits

KEGG orthology group: None (inferred from 82% identity to pfo:Pfl01_3473)

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcD" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W1L7 at UniProt or InterPro

Protein Sequence (312 amino acids)

>AO353_26685 cobalt efflux pump (Pseudomonas fluorescens FW300-N2E3)
MRSTPQVSRYSHDHMFLGATHDENARRTLWVVALTFVMMVGEIIAGYITGSMALLADGFH
MATHVGALGITAIAYGFARRNAGNARYSFGTGKVGDLAGFASAMVLGLVSIFIAVESVMR
LFQPTTVAFTEATLIAVLGLAVNIVSALLLAGNSAHHDHGHGHGHGHGHGHGHHHDNNLR
SAYVHVLADAMTSVLAIAALLAGRYLGWVWLDPVMGIVGAIVIANWAYGLMRDSAAVLLD
TTDEHVAAEVRELLESTGDVSITDLHVWRVGPQARAAIVSVVTSAAVTADTIRERLAPVH
ELSHLTVEYRNA