Protein Info for AO353_26600 in Pseudomonas fluorescens FW300-N2E3

Annotation: disulfide bond formation protein DsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 transmembrane" amino acids 35 to 57 (23 residues), see Phobius details PF00529: CusB_dom_1" amino acids 50 to 371 (322 residues), 38.2 bits, see alignment E=2.2e-13 PF13533: Biotin_lipoyl_2" amino acids 80 to 125 (46 residues), 26 bits, see alignment 1.3e-09 PF16576: HlyD_D23" amino acids 205 to 320 (116 residues), 53.9 bits, see alignment E=3.1e-18 PF13437: HlyD_3" amino acids 245 to 363 (119 residues), 53.5 bits, see alignment E=7e-18

Best Hits

KEGG orthology group: None (inferred from 88% identity to pfs:PFLU3955)

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WAU4 at UniProt or InterPro

Protein Sequence (383 amino acids)

>AO353_26600 disulfide bond formation protein DsbA (Pseudomonas fluorescens FW300-N2E3)
MTDPITTTTNAIASTPEGVAPPKTSGTEPRSLPVRIISSLGFAGIAIVGVLIVLYAWQLP
PFGSAIESTENALVRGQVTIIGPQLSGYVFEVPVQDFQFVKTGDLLVRLDDRIYKQRLDQ
SLAQLAVQKAALANAVQQRNSAEATIKLRQAALTDSQAQAQKSAADLRRNEALISDGSVS
KRELDVTRAANAQTIAAVAQAQASLEIARQDLQTVIVNRGSLEAAVASAEAAVELARIDL
SNTRVVAPQDGQLGQIGVRLGAYVNSGAQLMALVPSQLWVIANMKETQMNNIHIGQPVTF
TVDALNHQKFHGRVQRISPATGSEFSLLQADNATGNFVKIAQRVPVRITVDANQAQSERL
RPGMSVVVSIDTSGQGNAVADNP