Protein Info for AO353_26565 in Pseudomonas fluorescens FW300-N2E3

Annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 transmembrane" amino acids 49 to 73 (25 residues), see Phobius details amino acids 79 to 101 (23 residues), see Phobius details amino acids 126 to 151 (26 residues), see Phobius details amino acids 163 to 182 (20 residues), see Phobius details amino acids 194 to 212 (19 residues), see Phobius details amino acids 232 to 252 (21 residues), see Phobius details amino acids 272 to 294 (23 residues), see Phobius details amino acids 314 to 336 (23 residues), see Phobius details amino acids 357 to 374 (18 residues), see Phobius details amino acids 394 to 414 (21 residues), see Phobius details amino acids 435 to 454 (20 residues), see Phobius details amino acids 460 to 480 (21 residues), see Phobius details PF02133: Transp_cyt_pur" amino acids 38 to 471 (434 residues), 299.2 bits, see alignment E=2.5e-93

Best Hits

KEGG orthology group: K03457, nucleobase:cation symporter-1, NCS1 family (inferred from 91% identity to pfo:Pfl01_3440)

Predicted SEED Role

"Possible pyrimidine permease in reductive pathway" in subsystem Pyrimidine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X1L8 at UniProt or InterPro

Protein Sequence (495 amino acids)

>AO353_26565 nitrate reductase (Pseudomonas fluorescens FW300-N2E3)
MQQNRSHVTEREGLYELDAGSDVLDSPRYNHDIAPTKVHERTWNKWHITALWIGMSVCVP
TYTLGGVLTAYFGLSVGEALLAILLANVIVLIPLTLNAFAGTKYGIPFPVLLRSSFGVIG
SNVPCLIRALVACGWFGIQTMFGGLAIHLFLGSVFEGWKSLGGTGEVIGFMMFWTLNLWV
VLRGAESIKWLETLSAPLLVAVGLGLLVWAMPNVSLTELLAQPPKRPEGASVTGYFLAGL
TAMVGFWATLSLNIPDFSRYAKSQKDQILGQIFGLPLTMFLFASLGGVMTAASAKLVGVT
VSDPVSLIGHIQSPVWVAFAMALIIIATLSTNTAANIVSPTNDFQNLAPKLINRTKAVML
TGLVGLALMGHELLKKLGLIVSNVSLETVYSNWLLGYSSLLGPIAGIMVVDYFLIKKQQL
DLAGLYRDDVYPAWNWNGFIAFGVPVVLTLLSLGSDAFSWFYSYGWFTGSALGGLMYYGL
CTMRSSDTAVADTSI