Protein Info for AO353_25975 in Pseudomonas fluorescens FW300-N2E3

Annotation: carboxylate--amine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 PF14401: RLAN" amino acids 58 to 209 (152 residues), 181.1 bits, see alignment E=2.1e-57 PF08443: RimK" amino acids 318 to 495 (178 residues), 34.1 bits, see alignment E=4.3e-12 PF07478: Dala_Dala_lig_C" amino acids 345 to 492 (148 residues), 33 bits, see alignment E=8.8e-12

Best Hits

KEGG orthology group: None (inferred from 91% identity to pfo:Pfl01_2617)

Predicted SEED Role

"ribosomal protein S6 glutaminyl transferase related protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WPJ3 at UniProt or InterPro

Protein Sequence (528 amino acids)

>AO353_25975 carboxylate--amine ligase (Pseudomonas fluorescens FW300-N2E3)
MSALQGHWREVSEQRLPTAITKATYLSEAARTSSQLVIIVERKEDWASYFPSEDIVSAQE
YLEQTRGSEQGKRVQVINLCRSYKYLGHGYYCSLLAEARGHKVIPSVRTISELTRKSLYG
LSLDDLDKALDKALNNHLYSDTEGFTLTLYFGKTNIEPLQDLARQLFEVFPCPILLVEFR
RRNGWHIEGVKFGALHKLREDQEDHFANALDNFSRKIWRMPRSRRLARYDLAILHDPQEA
LPPSNARALENFVRVGKGLGIDVELIERKDYARIAEYDGLLIRETTSVDNHTYRFAKKAE
SEGLVVMDDPASILRCTNKVYLTDLLNSHQLGMPATEILYKERPQDFERVGERLGFPLVL
KIPDGCFSRGVIKVESQQALLEATAELFEHSVLLLAQEFFYTEYDWRIGVLNRKPIFACQ
YFMSKGHWQIYNHKAKGQDINGECRTLAVHEAPKAVVDLAVKTANLIGDGLYGVDLKQSG
DKVVVIEVNDNPNIDAGIEDAYLQDDLYSLVLEEFVRRLELKRRGQVW