Protein Info for AO353_25715 in Pseudomonas fluorescens FW300-N2E3

Annotation: multidrug DMT transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 transmembrane" amino acids 16 to 36 (21 residues), see Phobius details PF13473: Cupredoxin_1" amino acids 30 to 133 (104 residues), 70 bits, see alignment E=1.6e-23 PF09375: Peptidase_M75" amino acids 160 to 387 (228 residues), 91.6 bits, see alignment E=6.8e-30

Best Hits

Swiss-Prot: 42% identical to EFEO_YERPE: Iron uptake system component EfeO (efeO) from Yersinia pestis

KEGG orthology group: None (inferred from 79% identity to pfs:PFLU2920)

MetaCyc: 42% identical to ferrous iron transport system protein EfeO (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Ferrous iron transport periplasmic protein EfeO, contains peptidase-M75 domain and (frequently) cupredoxin-like domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WAI1 at UniProt or InterPro

Protein Sequence (399 amino acids)

>AO353_25715 multidrug DMT transporter permease (Pseudomonas fluorescens FW300-N2E3)
MSNQAPLQTSPPRALRRAVAGSVIVMIAAGGLFYYASQMAAAKRQTNHDEITVTINPHSC
EPNALTVPAGRSSFRIVNHSDRAVEWEILDGVLVLEERENIAPGLSQVITANLLPGDYAI
TCGLLSNPRGTLHVTPTAASDAAAQARPSIVAFIGPLSEFRVYLSSQGTALINAVSALQK
AIDAGDLSQAQALYVPARVAYQRLAPAAQRLAELDKVINARADYFEKREQDPQFVGFHRL
EYALFQQRNIDGLAPVAQRLVADVTQLKQQLLAQSLPPEQLVSIVARNLRNMADIRASSG
EEERYSHIDLNGFAANLDVTRKVVDLLRPLLTKPAAALLTRLDTATHALDAELNGLRVKG
GYSSYDGVGAAQRARLADKAKALADALDGIDPALGLSGL