Protein Info for AO353_25615 in Pseudomonas fluorescens FW300-N2E3

Annotation: 2-methylcitrate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 transmembrane" amino acids 177 to 195 (19 residues), see Phobius details PF03972: MmgE_PrpD_N" amino acids 6 to 245 (240 residues), 199.3 bits, see alignment E=6.1e-63 PF19305: MmgE_PrpD_C" amino acids 270 to 434 (165 residues), 108.8 bits, see alignment E=2.7e-35

Best Hits

KEGG orthology group: None (inferred from 78% identity to pfo:Pfl01_2858)

Predicted SEED Role

"Immune-responsive protein 1"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0W5 at UniProt or InterPro

Protein Sequence (449 amino acids)

>AO353_25615 2-methylcitrate dehydratase (Pseudomonas fluorescens FW300-N2E3)
MSERLQRLAQFCVETRFDDLPPALVAQAKRHILDTVGATLAGAASEIAVAVRHTFANENG
KTLVWGTAQQVGAAQAAMLNGIAAHALELDDTGGCDHSGAVVLPAVMAAVSLSPTPVNGR
ELITAVVIGYEIGRRVLEACGGYSAHNGAGWHSTATCGVFGAAAASARILGLDPAQTLAA
LGIAGSFSGGLWAFIHDGSHSKKLHSGRAAEGGLLAARFAEQGISGPTKLFDDVWGGFLK
TLATEHAQPEALDADLGVVWKLARCSIKPYASCRGTHSAIDALGLLLEQLQIDADQLEDV
QVSLCGFLNEMCGGRDIGSLAAAQMSLPYALAARLVHGHCRLEAYDDEQRSTSEIAHWMS
RIHLEVDPQLSEDGEPIVSIRSRDGRTASLCVEVPLGAPGNPLSDAALEEKFLSLATRVL
PVEQATGLLERLWQLEKLQSVGTLIEIIE