Protein Info for AO353_25585 in Pseudomonas fluorescens FW300-N2E3
Annotation: argininosuccinate lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 92% identical to ARLY1_PSEPF: Argininosuccinate lyase 1 (argH1) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 92% identity to pfo:Pfl01_2852)Predicted SEED Role
"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)
MetaCyc Pathways
- superpathway of arginine and polyamine biosynthesis (16/17 steps found)
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- nitric oxide biosynthesis II (mammals) (2/3 steps found)
- urea cycle (3/5 steps found)
- canavanine biosynthesis (2/4 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 4.3.2.1
Use Curated BLAST to search for 4.3.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VUG9 at UniProt or InterPro
Protein Sequence (475 amino acids)
>AO353_25585 argininosuccinate lyase (Pseudomonas fluorescens FW300-N2E3) MTQATDRLWGARFKSGPSEALAALSRCPERYFRLTPYDLAGSKAHAGELHRAGLLDALET QTMLDALERIGEDFRAGFVSPTLDDEDVHTFIERLLTERLGALGGKLRAGRSRNDQTAND LRLFLRDHARTLTTEVLALQQALVEQAELHIDSICPGFTHLQQAQPIVFAHHLLAHAQSM LRDVQRLVDWDVRTSLSPLGAAAMAGSAIARQPQQSAKEMGYSGVCENSIDAVASRDHVA EFLFVASMLGVNISRLAEEFCLWSSRQFRWVALDDAYATGSSIMPQKKNPDIAELARGKA GRLIGNLTGLLSTLKSLPLSYNRDLSEDKNGVLDSVDTLLLVLPAMAGMVATMKVNVEEL RRQAPLGFTLATEVADWLAVRGVPFKEAHEITGALVQACEKHDIELWEASPALLAEIDMR LTPEVRDSLTLEAAIAARSGWGGTAPQQVREQIGRLKTALAAQQRWAENYQGFRL