Protein Info for AO353_25540 in Pseudomonas fluorescens FW300-N2E3

Annotation: UDP-4-amino-4-deoxy-L-arabinose formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 PF00551: Formyl_trans_N" amino acids 27 to 177 (151 residues), 90 bits, see alignment E=3.4e-29 PF02911: Formyl_trans_C" amino acids 205 to 284 (80 residues), 59.9 bits, see alignment E=4.5e-20 PF01370: Epimerase" amino acids 321 to 568 (248 residues), 150.2 bits, see alignment E=1.4e-47 PF16363: GDP_Man_Dehyd" amino acids 322 to 645 (324 residues), 89 bits, see alignment E=8.7e-29

Best Hits

Swiss-Prot: 94% identical to ARNA_PSEF5: Bifunctional polymyxin resistance protein ArnA (arnA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K10011, UDP-4-amino-4-deoxy-L-arabinose formyltransferase / UDP-glucuronic acid dehydrogenase (UDP-4-keto-hexauronic acid decarboxylating) [EC: 1.1.1.305 2.1.2.13] (inferred from 94% identity to pfl:PFL_3045)

MetaCyc: 70% identical to fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-1862 [EC: 2.1.2.13]; RXN0-1861 [EC: 2.1.2.13, 1.1.1.305]

Predicted SEED Role

"UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13) / UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305)" (EC 1.1.1.305, EC 2.1.2.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.305 or 2.1.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WAG3 at UniProt or InterPro

Protein Sequence (663 amino acids)

>AO353_25540 UDP-4-amino-4-deoxy-L-arabinose formyltransferase (Pseudomonas fluorescens FW300-N2E3)
MSSKAVVFAYHDIGCAGIEALLNAGFEIAAVFTHADDPKENTFYGSVAQLCARKGIPVHA
PEDVNHPLWIERIAKLNPDYLFSFYYRNLLGEQLLATARKGAFNLHGSLLPTYRGRAPAN
WVLVNGETETGVTLHRMVKRADAGAIIAQQRVAIERADTALTLHGKLREAASNLLRDTLP
LLAQGKITETAQDESKASVYGRRTAADGKLVWKKPAEELFNLVRAVTQPYPGAFCAVGEH
KLIVWSAEVVKGNEGLAPGRVISVDPLRIACGEDSLVVTSGQRNENGLYLSGPQLANELG
LVDGSVLRGAESGRAPRRTRVLILGVNGFIGNHLSERLLRDDKYDVYGLDIGSDAIERLR
SHPNFHFVEGDISIHSEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKLV
RYCVKYNKRVIFPSTSEVYGMCQDKNFDEDTSNLIVGPINKQRWIYSVSKQLLDRVIWAY
GAKGLNFTLFRPFNWMGPRLDRLDSARIGSSRAITQLILNLVEGTPIRLFDGGEQKRCFT
DIADGIEALARIVDNENDSCNGQVINIGNPDNEASIRQLGEELLRQFEAHPLRANFPPFA
GFRDVESKAFYGAGYQDVEHRKPSIENAKRLLNWEPTVEMSETIGNTLDFFLREAMLEIA
DKR