Protein Info for AO353_25530 in Pseudomonas fluorescens FW300-N2E3

Annotation: UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 PF01041: DegT_DnrJ_EryC1" amino acids 12 to 374 (363 residues), 434.6 bits, see alignment E=6.8e-134 PF01053: Cys_Met_Meta_PP" amino acids 33 to 166 (134 residues), 29.1 bits, see alignment E=7.9e-11 PF00155: Aminotran_1_2" amino acids 36 to 163 (128 residues), 44 bits, see alignment E=3.7e-15 PF00266: Aminotran_5" amino acids 43 to 320 (278 residues), 29.3 bits, see alignment E=8.8e-11

Best Hits

Swiss-Prot: 90% identical to ARNB_PSEF5: UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K07806, UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase [EC: 2.6.1.87] (inferred from 90% identity to pfl:PFL_3043)

MetaCyc: 68% identical to UDP-4-amino-4-deoxy-L-arabinose aminotransferase (Escherichia coli K-12 substr. MG1655)
RXN0-1863 [EC: 2.6.1.87, 2.6.1.92]

Predicted SEED Role

"UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC 2.6.1.-)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) (EC 2.6.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.-

Use Curated BLAST to search for 2.6.1.- or 2.6.1.87 or 2.6.1.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WHP3 at UniProt or InterPro

Protein Sequence (379 amino acids)

>AO353_25530 UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase (Pseudomonas fluorescens FW300-N2E3)
MNQAFLPFSRPSIGDEEIAAVEQVLRSGWITTGPKNQELEEQFAQYTGCQHAVALSSATG
GMHITLLALGIGPGDEVITPSQTWVSTANMICLLGATPVFVDVDRDTLMTDVQAIEAAIT
PRTKAIIPVHYAGAAFDLDPLYALADKHGIAVIEDAAHAAGTRYRGRAIGAQGTAIFSFH
AIKNMTCAEGAMFVSDNQALADRVRMLKFHGLGVDAYDRLTHGRKPQAMVIEPGFKYNLA
DINAAIALVQLQRLDEINAKRTALAQTYLQRLEGLPVQPLSVPTYPQQHAWHLFILRIDA
ERCGMDREAFMKALQEQNIGTGIHFVATHLHTYYRQRFPGIHLPNTEWNSARLCSIPLFP
DMTTDDVERVVGAIENILD