Protein Info for AO353_25515 in Pseudomonas fluorescens FW300-N2E3

Annotation: iron ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01547: SBP_bac_1" amino acids 42 to 285 (244 residues), 77.5 bits, see alignment E=3.8e-25 PF13531: SBP_bac_11" amino acids 45 to 288 (244 residues), 66.6 bits, see alignment E=5.9e-22 PF13416: SBP_bac_8" amino acids 46 to 286 (241 residues), 83.7 bits, see alignment E=4e-27 PF13343: SBP_bac_6" amino acids 81 to 313 (233 residues), 87.6 bits, see alignment E=1.9e-28

Best Hits

Swiss-Prot: 72% identical to FBPA_SERMA: Fe(3+)-binding periplasmic protein (fbpA) from Serratia marcescens

KEGG orthology group: K02012, iron(III) transport system substrate-binding protein (inferred from 81% identity to pfl:PFL_3038)

Predicted SEED Role

"Ferric iron ABC transporter, iron-binding protein" in subsystem Campylobacter Iron Metabolism or Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VZT0 at UniProt or InterPro

Protein Sequence (338 amino acids)

>AO353_25515 iron ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2E3)
MNHRRPSFLKIALLATALMGAGHVFAAPSSDGIVVYNAQHETLTKAWVEGFTHETGIKVT
LRNGDDSEMGNQLVQEGAASPADVFLTENSPAMVLVDNAGLFTPVAPSTLEQIGSAYRPA
HGKWVGIAARSTVFVYNKAKLAEADLPKSLMDLASPSWKGRWAGSPAGADFQAIVAAVLE
LKGEAATLEWLKGMKANATAYRGNSAVLKAVNAGQIEGGVIYHYYSFVDQSKTGENSKNT
SLHYFKHKDPGAFVSLSGGAVLASSKHQEEAQAFLKWITGKEGQAILKNGNSFEYAVGQK
AESNPKLVPLAQLDAPVVDASRLNSKKVVELMTQAGLL