Protein Info for AO353_25490 in Pseudomonas fluorescens FW300-N2E3

Annotation: transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 230 transmembrane" amino acids 64 to 84 (21 residues), see Phobius details PF00072: Response_reg" amino acids 4 to 114 (111 residues), 81.5 bits, see alignment E=5e-27 PF00486: Trans_reg_C" amino acids 152 to 226 (75 residues), 94.7 bits, see alignment E=2.9e-31

Best Hits

Swiss-Prot: 46% identical to PRRA_MYCTU: Transcriptional regulatory protein PrrA (prrA) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K02483, two-component system, OmpR family, response regulator (inferred from 59% identity to gdj:Gdia_2157)

Predicted SEED Role

"DNA-binding heavy metal response regulator" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VZ96 at UniProt or InterPro

Protein Sequence (230 amino acids)

>AO353_25490 transcriptional regulator (Pseudomonas fluorescens FW300-N2E3)
MTHVLVVEDDSSTAREIEAALQDHGFTVTCVNNGRDGLLKALSEPFDLIVLDRMLPGGID
GLGVLTALRAASVSTPVLILSALSALDERVRGLRAGGDDYLTKPFEFIELTARLDALSRR
RAEPATREPDNQLRVGNLHVDLLRRSVRRGDRTIDLVPREYALLEHLMRNAEQVVTRTML
FEAVWNYSYDERTNVIEVHIGRLRRKIDAEGEPQMIHTIRGAGYVLRSPE