Protein Info for AO353_25480 in Pseudomonas fluorescens FW300-N2E3

Annotation: multidrug transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 19 to 448 (430 residues), 311.3 bits, see alignment E=5.8e-97 PF02321: OEP" amino acids 66 to 246 (181 residues), 76.7 bits, see alignment E=1e-25 amino acids 276 to 447 (172 residues), 76.7 bits, see alignment E=1.1e-25

Best Hits

KEGG orthology group: None (inferred from 37% identity to pla:Plav_1099)

Predicted SEED Role

"RND efflux system, outer membrane lipoprotein CmeC" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WAF6 at UniProt or InterPro

Protein Sequence (466 amino acids)

>AO353_25480 multidrug transporter (Pseudomonas fluorescens FW300-N2E3)
MSVATSPRRNFLSLSVLLGVLMLGGCSMVPAYHQPELPLAANWKGTGQTSAARGGQWWSE
FHSAELNDLITRGLSANYTLKAAASRIEEARASAQVVGAGQYPTLALGGNVQRQNNYGTT
KKSSVFAQATYELDFWGKQRAAADSADALANASVFDAQTLRMGLGASIADSYFQVLSLEE
RLRLAQSIADDAKQVLDLVEVQASQGAVSNLEVAQQRNALQTFQAAMPPLRQQRDLALYQ
LAVLVGETPQGFHLKSSGLDGLQMPRPSAGLPIGLLRQRPDVQAAEARLVSANFDVGVAR
AAFLPNLSLDLIGGIDTIAGGNIWSVVGTLTQPVFTGGQLTGQLHVDQAHVQELTASYRE
SVIEALQDVETQLSATRELDKSFALNQAAVDSAREAARLAQVRYRLGSTDFQTLLIVERT
QYQAEDTLLQVRLQHLQAAVGLFRALGGDFTPDTLASLSPSQASHP