Protein Info for AO353_25135 in Pseudomonas fluorescens FW300-N2E3

Annotation: glycosyl hydrolase family 32

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 TIGR01322: sucrose-6-phosphate hydrolase" amino acids 21 to 459 (439 residues), 450.8 bits, see alignment E=2.4e-139 PF00251: Glyco_hydro_32N" amino acids 28 to 329 (302 residues), 349.2 bits, see alignment E=2.6e-108 PF08244: Glyco_hydro_32C" amino acids 357 to 477 (121 residues), 96.2 bits, see alignment E=2.1e-31

Best Hits

KEGG orthology group: K01193, beta-fructofuranosidase [EC: 3.2.1.26] (inferred from 85% identity to pba:PSEBR_a2364)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.26 or 3.2.1.B3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VZ31 at UniProt or InterPro

Protein Sequence (489 amino acids)

>AO353_25135 glycosyl hydrolase family 32 (Pseudomonas fluorescens FW300-N2E3)
MSSSLDLAQRALSDGLSRVIHDYRPGYHIAPPAGWMNDPNGVVYFRGEYHVFYQHYPFEA
KWGPMYWGHAKSADLVHWQHLPIALAPGDDFDRDGCFSGSAVVCGDTLALIYTGHTWLGE
AGDERSIRQVQCLATSIDGIRFVKHGAVIETPPQDTIMHFRDPKVWKEDDCWYLIAGARL
GDIPLLPLYRSTDLHAWEFLGYVSGGSEGDGYMWECPDLFRLNGRDVLLYSPQGMQPQGY
ERLNKYQTGYRVGRLDSEWHFTGGPFIELDNGHDFYAAQTLLAADGRRLLWAWLDMWESP
MPSKAHHWCGMLGLPRELELRADRLCVYPARELTALRKAPLPGTPWWDESGTRWVPEVKG
DMLEIHVHLDLLGCTDGHLGIALRCSDDGYEETLLYYDASLQRLVLDRSRSGAQATGQRS
VSIDPTQERLELRVFLDRSSIEVFDENGRFSLSSRLYPQPGSLGVKLLASGSGGRVSIPR
AWPLASGWL