Protein Info for AO353_25065 in Pseudomonas fluorescens FW300-N2E3

Annotation: tricarballylate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR02485: precorrin 3B synthase CobZ" amino acids 2 to 466 (465 residues), 665.4 bits, see alignment E=1.8e-204 PF12831: FAD_oxidored" amino acids 3 to 166 (164 residues), 37.6 bits, see alignment E=5.5e-13 PF00890: FAD_binding_2" amino acids 3 to 441 (439 residues), 107.6 bits, see alignment E=2.8e-34

Best Hits

KEGG orthology group: K13796, tricarballylate dehydrogenase (inferred from 90% identity to pba:PSEBR_a2045)

MetaCyc: 75% identical to propane-1,2,3-tricarboxylate dehydrogenase (Salmonella enterica enterica serovar Typhimurium str. LT2)
1.3.8.M1 [EC: 1.3.8.M1]

Predicted SEED Role

"TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate" in subsystem Tricarballylate Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.M1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WSL1 at UniProt or InterPro

Protein Sequence (483 amino acids)

>AO353_25065 tricarballylate dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MIDVLVIGGGNAALCAALMAREAGASVMLLEGAPKIWRGGNSQHTRNLRCMHDAPQDVLV
DAYPEEEYWQDLLKVTGGITNEKLARIVIRASSNCRDWMRSHGVHFQPSLSGALHVARTN
AFFMGGGKALVNSYFRSAERLGVKIRYNAQVTDIEMKGGEFVAAYIGEREVDGQPLPAER
IQAKACVLAAGGFESNREWLREAWGQNERGEWPSDNFLIRGTRFNTGVLLRRMQSLGADF
IGDPTQAHMVAIDARAPLYDGGICTRIDCVSLGVVVNRDGERFYDEGEDFWPKRYAIWGR
LVAGQPGQQAYSIIDQQALGRFMPPVFPGTKANSLQELARQLKLPVTQFVKTIEEYNAAC
RIGTFDHTALDDCHTEGLTPAKTHWARPLVKPPFCAYPLKPGVTFTYLGLATDDTAAVHF
NGEASPNLFVAGEMMAGNVLGKGYTAGIGMAIGTAFGRIAGVQAAAAAGFLTPSSTEPQH
AAV