Protein Info for AO353_25060 in Pseudomonas fluorescens FW300-N2E3

Annotation: LysR family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 transmembrane" amino acids 89 to 108 (20 residues), see Phobius details PF00126: HTH_1" amino acids 3 to 61 (59 residues), 68.8 bits, see alignment E=3.1e-23 PF03466: LysR_substrate" amino acids 85 to 285 (201 residues), 125 bits, see alignment E=2.8e-40

Best Hits

KEGG orthology group: K13794, LysR family transcriptional regulator, regulatory protein for tcuABC (inferred from 62% identity to vap:Vapar_3132)

Predicted SEED Role

"TcuR: regulates tcuABC genes used in utilization of tricarballylate" in subsystem Tricarballylate Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0V2 at UniProt or InterPro

Protein Sequence (311 amino acids)

>AO353_25060 LysR family transcriptional regulator (Pseudomonas fluorescens FW300-N2E3)
LELRQLRYFVKILEHGSLGRAALELDVGVSALSQQIAKLESELSTRLLNRTTTGVTPTSS
GMAFLHHAQLCLRQAENAIMAAHRGRMSGYVTVGMAPTTATVLALPLINAMRERYPDIQL
HLVEMLSGHLATQLNARQIDLAILFQLEGGKRWSVTPLLDEKLFVISSPHLPHAPQGTSV
HLADLGQLPLVMPSAQHGLRSTLMVAFERSGLAPNIIMEVDGLAVLMNAVRAGHAATIQP
GAAAALKGESGLSVVEISDAHVGRRNLLATLPDDELSPAALAARLVITDVARQLVVDQHW
PGASWIEDVAS