Protein Info for AO353_25005 in Pseudomonas fluorescens FW300-N2E3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 43 to 63 (21 residues), see Phobius details amino acids 75 to 99 (25 residues), see Phobius details amino acids 120 to 140 (21 residues), see Phobius details amino acids 142 to 157 (16 residues), see Phobius details amino acids 185 to 207 (23 residues), see Phobius details amino acids 214 to 232 (19 residues), see Phobius details amino acids 253 to 276 (24 residues), see Phobius details amino acids 283 to 302 (20 residues), see Phobius details amino acids 310 to 329 (20 residues), see Phobius details PF01699: Na_Ca_ex" amino acids 5 to 156 (152 residues), 50.7 bits, see alignment E=1e-17 amino acids 189 to 326 (138 residues), 60.4 bits, see alignment E=1.1e-20

Best Hits

KEGG orthology group: K07301, inner membrane protein (inferred from 60% identity to bgd:bgla_2g24440)

Predicted SEED Role

"Ca2+/Na+ antiporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W1Y5 at UniProt or InterPro

Protein Sequence (332 amino acids)

>AO353_25005 hypothetical protein (Pseudomonas fluorescens FW300-N2E3)
MPMTFALLLGSAVVIYLACEYFVNGIEWVGRKLAVGQQATGSILAAFGTALPESVITFIA
VVFGDTPEQKSLGVGAALGGPLVLATIAYAVVGVTLLLCRQRLANTEAIRQDFKRLSRDQ
GWFLAIFVAKIALGLVVFAYKPWLGILFLAAYAAYFWKEMRGEQDEEEYELEPLKLAPGK
ATPSTSAAVIQTLIALAVIFAASHFFVGQLDALGPMLGIHPQLLALLLSPIATELPETLN
AIIWVRQGKHRLALANISGAMMIQATVPTAFGLFFTPWALDTSLVLAAIITLIGVGIMFF
AFRKGIISRWLLASMVLLYILFAVLLKVLDLA