Protein Info for AO353_24935 in Pseudomonas fluorescens FW300-N2E3

Annotation: cysteine sulfinate desulfinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01979: cysteine desulfurase, SufS family
  • amino acids 25 to 424 (400 residues), 603.5 bits, E=9e-186
• PF00266: Aminotran_5
  • amino acids 43 to 412 (370 residues), 524.8 bits, E=3.1e-161
• PF00155: Aminotran_1_2
  • amino acids 50 to 220 (171 residues), 33.6 bits, E=6.6e-12
• PF01212: Beta_elim_lyase
  • amino acids 104 to 262 (159 residues), 30.2 bits, E=7.1e-11
• PF01041: DegT_DnrJ_EryC1
  • amino acids 109 to 223 (115 residues), 21.9 bits, E=2.4e-08

Best Hits

Swiss-Prot: 58% identical to SUFS_DICD3: Cysteine desulfurase (sufS) from Dickeya dadantii (strain 3937)

KEGG orthology group: K11717, cysteine desulfurase / selenocysteine lyase [EC: 2.8.1.7 4.4.1.16] (inferred from 68% identity to nhl:Nhal_0691)

MetaCyc: 53% identical to L-cysteine desulfurase (Escherichia coli K-12 substr. MG1655)
Selenocysteine lyase. [EC: 4.4.1.16]; Cysteine desulfurase. [EC: 4.4.1.16, 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), SufS subfamily" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of L-alanine biosynthesis (4/4 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• molybdopterin biosynthesis (4/6 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (4/10 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (4/11 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)

KEGG Metabolic Maps

• Selenoamino acid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7 or 4.4.1.16

Sequence Analysis Tools

See A0A0N9X0P4 at UniProt or InterPro

Protein Sequence (424 amino acids)

>AO353_24935 cysteine sulfinate desulfinase (Pseudomonas fluorescens FW300-N2E3)
MSDLENRLQGLSKPLHLTPGYDVERVRQAFPILAETIYGKPLIYLDSAATSQKPQAVIDA
MSHFFSKENANVHRGVHYLSVRATEEYEKARAKVQGFLNTESVEEIVFVRGTTEAVNLVA
QTYGKTQLRTGDEVLITAMEHHSNIVPWQMLCEQTGARLRVAPINDAGELLLDELERLIG
PRTRLVAVTHVSNVLGTINPIRRIVELAHARGVRVLVDGAQAAPHLKVDVQALGCDFYAL
SGHKMYGPTGIGVLYGRHELLESMPPYQGGGDMILSVSFEKTVYNKPPYKFEAGTPNMAG
AIGLGAAIDFLAELGPGAIAAHEQAVQAYATKALAALPGLRLIGTAAEKVGVLSFVLDGI
HPHDIGTILDREGIAIRTGHHCAQPLMNRFGVPATARASLGCYSTERDIDALVAGLAKVQ
EVFR