Protein Info for AO353_24920 in Pseudomonas fluorescens FW300-N2E3

Annotation: cysteine desulfurase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 TIGR01980: FeS assembly protein SufB" amino acids 15 to 473 (459 residues), 638.5 bits, see alignment E=2.5e-196 PF19295: SufBD_N" amino acids 133 to 203 (71 residues), 43 bits, see alignment E=4.1e-15 PF01458: SUFBD" amino acids 220 to 453 (234 residues), 241.7 bits, see alignment E=7.5e-76

Best Hits

Swiss-Prot: 73% identical to Y074_SYNY3: UPF0051 protein slr0074 (slr0074) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K09014, Fe-S cluster assembly protein SufB (inferred from 79% identity to nhl:Nhal_0688)

Predicted SEED Role

"Iron-sulfur cluster assembly protein SufB" in subsystem Staphylococcal phi-Mu50B-like prophages

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VZ06 at UniProt or InterPro

Protein Sequence (482 amino acids)

>AO353_24920 cysteine desulfurase (Pseudomonas fluorescens FW300-N2E3)
MSTDNARIRELSNRAYKYGFVSDFESDAAPRGLNEDLIRLISTKKNEPQWLLDWRLKAYR
HWLTMREPSWQNVHYAPIDYQDIIYYSAPKPKTPGPGSLDEVDPELREMFGKLGISLAEQ
ERLTGVAVDAVVDSVSVATTFKHKLAEVGVIFCSFSEAVQNHPELVRKYLGSVVPYSDNF
FATLNSAVFSDGSFCYVPKGVRCPMELSTYFRINAADTGQFERTLIVAEEGAYVSYLEGC
TAPMRDNNQLHAAVVELVALDNAQIKYATVQNWYPGDAQGRGGIFNFVTKRGKCAGAHSK
ISWTQVETGSAITWKYPGVILQGDYSVGEFYSVALTANHQQADTGTKMIHIGRHTRSTIL
SKGISAGHGQNTYRGLVKIQKAATGARNHTQCDSLLLGSECGAHTFPYIDVKNPSAQVEH
EASTSKISDEQLFYCRQRGISAEDAVPMIVNGFCREVLKELPMEFAVEAQKLLGVSLEGC
VG