Protein Info for AO353_24825 in Pseudomonas fluorescens FW300-N2E3

Annotation: amino acid transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 48 to 69 (22 residues), see Phobius details amino acids 88 to 109 (22 residues), see Phobius details amino acids 129 to 151 (23 residues), see Phobius details amino acids 163 to 184 (22 residues), see Phobius details amino acids 204 to 225 (22 residues), see Phobius details amino acids 246 to 267 (22 residues), see Phobius details amino acids 292 to 317 (26 residues), see Phobius details amino acids 337 to 359 (23 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details amino acids 408 to 430 (23 residues), see Phobius details amino acids 437 to 455 (19 residues), see Phobius details PF00324: AA_permease" amino acids 20 to 453 (434 residues), 426.6 bits, see alignment E=1.2e-131 PF13520: AA_permease_2" amino acids 21 to 442 (422 residues), 136.2 bits, see alignment E=1.6e-43

Best Hits

Swiss-Prot: 54% identical to ROCE_BACSU: Amino-acid permease RocE (rocE) from Bacillus subtilis (strain 168)

KEGG orthology group: K02205, arginine/ornithine permease (inferred from 88% identity to ppu:PP_3727)

MetaCyc: 49% identical to CP4-6 prophage; S-methyl-L-methionine transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-486

Predicted SEED Role

"Arginine permease RocE" in subsystem Arginine and Ornithine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0U7 at UniProt or InterPro

Protein Sequence (470 amino acids)

>AO353_24825 amino acid transporter (Pseudomonas fluorescens FW300-N2E3)
MSESIERKGIHLTRALKSRHIFMLSLGGVIGTGLFMGSGVTINQGGPVGAILAYLIAGFL
MYLVMVCLGELSVQMPVSGSFQTHATKFIGPATGFMIGWVYWMSWASTVGLEFTAAGMLM
VRWFPTVPIWYWSALFVVVLFGLNALATRAFGEAEYWFSGIKVAAILGFIVVGVLVIFGA
IPLTSGAPAPMMSNLIGDSLFPNGLPAVFAVMMTVVYAFQGCEIMGVAAGETDQPEKSIP
RAVRNVVFRVLIFYVLAIVVLSAIVPWQQAGLMESPFVQVFDMVGIPYAADLMNFVILTA
ILSVGNSGLYASTRILWAMSKTGMAPKSLSPLSKRGVPLRALSITLCFALVSLMTSFIAA
DTLFMVLMAVSGMSGTVTWIVIALAQYKFRRAYIRDGGKLSDLKYRAPWFPLLPILCIAL
CCSLFVFLAMDETQRPSLYWGFGFMALCYGAYFLIQRKRQAVLAPSLPTV