Protein Info for AO353_24800 in Pseudomonas fluorescens FW300-N2E3

Annotation: spermidine/putrescine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF13416: SBP_bac_8" amino acids 59 to 307 (249 residues), 161 bits, see alignment E=8.3e-51 PF01547: SBP_bac_1" amino acids 61 to 286 (226 residues), 38.7 bits, see alignment E=1.9e-13 PF13343: SBP_bac_6" amino acids 132 to 303 (172 residues), 37.1 bits, see alignment E=3.7e-13

Best Hits

KEGG orthology group: K02055, putative spermidine/putrescine transport system substrate-binding protein (inferred from 83% identity to pfl:PFL_2704)

Predicted SEED Role

"ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WNY4 at UniProt or InterPro

Protein Sequence (349 amino acids)

>AO353_24800 spermidine/putrescine ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2E3)
MKSNTLKHGFYPLLLTLAIGLGNAQAAPEMVVVGYGGAGQKAQDVAFFQPFSTADRSKLI
QSEYNGEMARIKVMVDTGNVDWDVVQIEGPDLMRGCDEGMYERLDWKSLGHAEQLIPEAA
QECGSAALVWSVAIAYDTQKLAQAPTSWADFWDVQKFPGKRGLRKRAVYNLEFALLADGV
KVEDVYKVLSTPQGVDRAFAKLTELKPHIQWWEAGAQPAQWLTAGDVVMTSTYSGRVAAA
AQSGSHLGLVWPGSLYGMDYWAIIKGSRHVDQAKRFIAFANQPDAQVKYVEQIPYGPTNT
QAAARLDSKLAQWVPTAPQNLKGALSMNVAFWVDHGEELEERFNAWASK