Protein Info for AO353_24755 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATP-dependent DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 754 PF00270: DEAD" amino acids 192 to 280 (89 residues), 27.8 bits, see alignment E=3e-10 PF06733: DEAD_2" amino acids 270 to 389 (120 residues), 58.7 bits, see alignment E=9e-20 PF13307: Helicase_C_2" amino acids 590 to 749 (160 residues), 133.7 bits, see alignment E=1.1e-42

Best Hits

KEGG orthology group: None (inferred from 82% identity to pfo:Pfl01_2690)

Predicted SEED Role

"DinG family ATP-dependent helicase CPE1197" in subsystem DNA repair, bacterial DinG and relatives

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X0L7 at UniProt or InterPro

Protein Sequence (754 amino acids)

>AO353_24755 ATP-dependent DNA helicase (Pseudomonas fluorescens FW300-N2E3)
VSYSVAVRALCEFTAKVGDLDLRFTPSPTALEGIVGHRTVASRRSETYQNEVALEGEYRQ
LTVRGRADGYDPDQNRLEEIKTYRGELERMPVNHRQLHWAQAKIYGWLMCQKLQLAEIKL
ALVYFDIVSEKETSLVETWSALELEHFFTLQCGLFLQWAEQEMAHREARNQAAQVLGFAY
PDFRPGQRHLAESVFKAVSTGRCLMAQAPTGIGKTVGTLFPMLKAFAPQQLDKVFFLTAK
TPGRKLALDAAQVIVDNSDGLPLRVLEMIARDKACEHLDKACHGESCPLAKGFYDRLPAA
REAASRVTLLDQAALREIALEHQVCPYYLSQEMARWADVVVADYNYYFDFSALLFGLAQA
NQWKVAVLVDEAHNLVERGRQMYSASLDQFALNTLRKSAPEVLKKSLQRVNREWNALHKT
QISPYQAYAKAPEKLLQALSLCTTAIGDYLNDHPQGLDAAVQSFYFDALQFSRVAELFDE
HFLFDISKRDLGNTRSLSQLCLRNVVPAGFIRPRLTAARSTVMFSATLSPQRYYADLLGL
PATTVWIDVESPFNAEQLKVQIVSRISTRFVHRQASLAPIVELMARQFIERPGNYLAFFS
SFDYLQQVAQLFAETWPQINLWSQSRGMDEARRQAFLEQFTPTSQGIGFAVLGGAFGEGI
DLPGSRLIGAFIATLGLAQLNPVNEQLKQRMAAIFDAGYDYTYLFPGVQKVVQAAGRVIR
TQQDQGVVMLIDDRFGESKVQQLLPRWWSVSGRA