Protein Info for AO353_24725 in Pseudomonas fluorescens FW300-N2E3

Annotation: polyurethanase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 PF00353: HemolysinCabind" amino acids 317 to 344 (28 residues), 28.1 bits, see alignment (E = 8e-11) amino acids 319 to 346 (28 residues), 25.3 bits, see alignment (E = 5.9e-10) amino acids 338 to 364 (27 residues), 8 bits, see alignment (E = 0.00015) amino acids 440 to 467 (28 residues), 14.3 bits, see alignment (E = 1.7e-06) amino acids 473 to 492 (20 residues), 15.5 bits, see alignment (E = 7.1e-07)

Best Hits

KEGG orthology group: K01046, triacylglycerol lipase [EC: 3.1.1.3] (inferred from 74% identity to pfo:Pfl01_2685)

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.3

Use Curated BLAST to search for 3.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WLA3 at UniProt or InterPro

Protein Sequence (564 amino acids)

>AO353_24725 polyurethanase (Pseudomonas fluorescens FW300-N2E3)
MGVFDYRKFGSEASKALYSDAIALTLYAYTPTGKPLATGWTPVTAGQLGYQGKVGAQGTF
FGEQEGFTSAEAEVLAKYDAAGKLISLGIAFRGTGGLGYSDTYGDMKNNLLAAIGPKDYA
KNYAKSAFDTLLKDVAAFASAKGLNAKDVLITGHSLGGLGANSMAELSADHWGGYFKDAN
YMTFASPTQSPAGTHVLNIGYENDPVFRVLDGTTFTSSSLYKHDKVQDSATNNIVNVNDH
YVSTQMTPFSIANPQHWAAHSSLGYADGLNRVIGSSFYDLTSKDSTILVSTLSESLRAST
WIEDLGRSGETHTGSTFIIGTDSNDLLRGSAGNDFLEGRDGNDTFRDAGGYNILLGGKGQ
NVFDLQQPLKNFTFANDGDGTLYVRDSYGGISMTRDIGALVSKESGAMWGLLNKDVTYNV
SAKGLLAGTELTHYSHSLNGDAYGNSLVATADGDWLFGLSGNDVLRSDKSQVTFVGGEGN
DVMYASGGHNTFLFSGAFGFDAINGYQGSDKLVFMGVQGAGQGYDYSQHVSQSGNDTLLK
VGDYAVTLVGVGLNNLSGAEIIFA