Protein Info for AO353_24710 in Pseudomonas fluorescens FW300-N2E3

Annotation: peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01844: type I secretion outer membrane protein, TolC family" amino acids 30 to 447 (418 residues), 393.5 bits, see alignment E=5.9e-122 PF02321: OEP" amino acids 30 to 221 (192 residues), 82.7 bits, see alignment E=1.6e-27 amino acids 248 to 433 (186 residues), 101.3 bits, see alignment E=2.9e-33

Best Hits

Swiss-Prot: 53% identical to APRF_PSEAE: Alkaline protease secretion protein AprF (aprF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12538, outer membrane protein HasF (inferred from 85% identity to pfo:Pfl01_2682)

Predicted SEED Role

"ABC-type protease exporter, outer membrane component PrtF/AprF" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VU41 at UniProt or InterPro

Protein Sequence (454 amino acids)

>AO353_24710 peptidase (Pseudomonas fluorescens FW300-N2E3)
MFRRMSKHSILGTAIALLVCTNVYAMGPFEVYEQALRNDPIFLGAIKERDAGLENRTIGR
AGLLPKLSYNYNKGRNNSKATSLDERRRNQTEDRNYNSYGSTFTLQQPLFDYEAYAAYRK
GVAQSLFADENFRGKSQELLVRVLTYYTQALFAQDQIDIAQAKKKAFEQQFQQNEQMFRQ
GEGTRTDILEAESRYELATAEEIEARDEQDASLRELGALIGTRDIDIKDLEPLSESFESF
SLQPANFDSWHELALSNNPNLASQRQAVEVARFEVERNRAGHLPRVSAYATARKNESESG
NTYNQRYDTNTIGIEISVPLYAGGGVSASTRQASRSMEQAEYDLDSKTRETLIELRRQFS
ACLSGVSKLRAYQKALTSAEALVVSTRQSILGGERVNLDALNAEQQLYTTRRDLAQARYD
YLMAWTKLHYYAGNLREEDLAKVDEAFGARKTTR