Protein Info for AO353_24700 in Pseudomonas fluorescens FW300-N2E3

Annotation: peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 597 transmembrane" amino acids 21 to 44 (24 residues), see Phobius details amino acids 60 to 85 (26 residues), see Phobius details amino acids 129 to 150 (22 residues), see Phobius details amino acids 156 to 175 (20 residues), see Phobius details amino acids 252 to 282 (31 residues), see Phobius details TIGR01842: type I secretion system ATPase" amino acids 18 to 558 (541 residues), 785.7 bits, see alignment E=1.1e-240 PF00664: ABC_membrane" amino acids 25 to 283 (259 residues), 43.3 bits, see alignment E=3.8e-15 PF00005: ABC_tran" amino acids 349 to 496 (148 residues), 108.7 bits, see alignment E=3.6e-35

Best Hits

Swiss-Prot: 59% identical to APRD_PSEAE: Alkaline protease secretion ATP-binding protein AprD (aprD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12536, ATP-binding cassette, subfamily C, bacterial HasD (inferred from 90% identity to pfo:Pfl01_2680)

Predicted SEED Role

"ABC-type protease exporter, ATP-binding component PrtD/AprD" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0U4 at UniProt or InterPro

Protein Sequence (597 amino acids)

>AO353_24700 peptidase (Pseudomonas fluorescens FW300-N2E3)
MRMAKTTPTAPLLKALGDYKSILISIGCFTALINVLMLVPSIYMLQVYDRVLSSQNETTL
VMLSLMVVGFFAFIGLLETVRSFIVIRIGSQLERRFNLRVYQAAFERNLFKGEGNAGQSL
GDLTHIRQFVTGPALFAFFDAPWFPVYLFVIYLFNVWLGVFATVGAVLLIGLAFLNEAIT
KKPLGEAGGYSQRSTQLATSHLHNAETIQAMGMLGVLRKRWFQVHSRFLGLQNQASDTGA
VISSLSKTLRLCLQSLVLGLGALLVIKGDMTAGMMIAGSILMGRVLSPIDQLIAVWKQWS
GAKLAYRRLDALLQEFPPNEDAMELPAPKGQVTFEQVSAGPPGKRAATLHMVNFNLGAGE
VLGVLGASGSGKSTLARVLVGVWPTLAGTVRLDGADIHRWNRDDLGPYIGYLPQDIELFS
GSVAENIARFREADPQKVVEAAQQAGVHEMILRLPQGYDTVLGEDGSGLSGGQKQRVALA
RALYGKPSLVVLDEPNSNLDTVGEAALASAIAQMKAQGTSVILVTHRSSALAQADKLLVL
NEGRLQAFGPSQEVLKALSGAQQSGAQQEQPREKTVAAPAGLSMSRQYQASTKSSGV