Protein Info for AO353_24690 in Pseudomonas fluorescens FW300-N2E3

Annotation: serine 3-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 PF00413: Peptidase_M10" amino acids 101 to 195 (95 residues), 26.1 bits, see alignment E=1.8e-09 PF13688: Reprolysin_5" amino acids 154 to 230 (77 residues), 27.4 bits, see alignment E=8.7e-10 PF13583: Reprolysin_4" amino acids 161 to 238 (78 residues), 31.5 bits, see alignment E=3.6e-11 PF08548: Peptidase_M10_C" amino acids 258 to 473 (216 residues), 252.7 bits, see alignment E=6.5e-79 PF00353: HemolysinCabind" amino acids 342 to 353 (12 residues), 7.5 bits, see alignment (E = 0.0011) amino acids 352 to 385 (34 residues), 32.5 bits, see alignment (E = 1.6e-11)

Best Hits

Swiss-Prot: 79% identical to APRA_PSEPA: Metallopeptidase AprA (aprA) from Pseudomonas panacis

KEGG orthology group: None (inferred from 81% identity to pfo:Pfl01_2678)

Predicted SEED Role

"Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog" in subsystem Protein secretion by ABC-type exporters (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X0K5 at UniProt or InterPro

Protein Sequence (474 amino acids)

>AO353_24690 serine 3-dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MSKVKVNAIDSLAATSSAWSQIDSFSHQYDRGGNLTVNGKPSYSVDQAATQLLRDGYAWH
DLNGSGKIELTYTFLTAPTSNFSGLGVTGFSQFSALQKSQAVLAMQSWGDVANVTFTEAA
KGGDGHMTFGNYSGGQEGAAAFAFLPGSEPGYDGQSWYLTGSGYNVNKTPGVNNYGRQTL
THEIGHTLGLSHPGDYNAGEGTPSYNDAVYGQDTRGYSLMSYWSESNTNQNFSKGGVEAY
SSGPLMDDIAAVQKLYGANMSTRTGDTTYGFNSNAGRDFYSATSSSDKVVFSVWDAGGKD
TLDFSGFTQNQKINLNGASFSDVGGMVGNVSIAKGVTIENAIGGSGNDLLIGNSVANELK
GGAGSDIIYGAGGADKLWGGSGSDTFVFAASSDSKPGAADQILDFTSGLDKIDLSAITNG
AGLHFVNSFTGAAGDAVLSSISGGSSLAIDFSGHGVADFLVTTVGQAATTDIVA