Protein Info for AO353_24495 in Pseudomonas fluorescens FW300-N2E3

Annotation: malate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 transmembrane" amino acids 25 to 48 (24 residues), see Phobius details amino acids 54 to 76 (23 residues), see Phobius details amino acids 85 to 110 (26 residues), see Phobius details amino acids 122 to 143 (22 residues), see Phobius details amino acids 150 to 172 (23 residues), see Phobius details amino acids 179 to 206 (28 residues), see Phobius details amino acids 214 to 238 (25 residues), see Phobius details amino acids 272 to 294 (23 residues), see Phobius details amino acids 298 to 318 (21 residues), see Phobius details amino acids 329 to 352 (24 residues), see Phobius details amino acids 360 to 383 (24 residues), see Phobius details amino acids 395 to 411 (17 residues), see Phobius details amino acids 426 to 446 (21 residues), see Phobius details PF03390: 2HCT" amino acids 30 to 441 (412 residues), 504.2 bits, see alignment E=1.2e-155

Best Hits

Swiss-Prot: 59% identical to MAEN_BACSU: Na(+)-malate symporter (maeN) from Bacillus subtilis (strain 168)

KEGG orthology group: K11616, malate:Na+ symporter (inferred from 68% identity to ddd:Dda3937_02539)

Predicted SEED Role

"Citrate-sodium symport"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WN27 at UniProt or InterPro

Protein Sequence (447 amino acids)

>AO353_24495 malate permease (Pseudomonas fluorescens FW300-N2E3)
MNKSYEQSLKIDAPRDGRLRQLRSLCGYEIGVIPLPIFLGIAVIVYLSAHLGFLPNNMIG
GLAVIMTMGMFFGQLGSRLPILKEIGGGAILCLMLPSILVFYGFFGPATIDATKMLMKEA
NFLYFVIASLVVGSILGMSRFILIQGMMRMFIPLLVGTLAAVASGLIVGKLVGYSFYHTF
FFIIVPIIGGGIGEGILPLSLAYSAILGGTPDIYVAKLVPAAVVGNIAAIICAGYLARLA
LKRPHLNGEGSLIRAKDENDKFQAREDNGSTIDFRVIGAGILVICAFFVLGGLLEKILGV
PGPVLMILAAVLFKYIRVLPEKLEKGTNAFYKLISSAFIWPVMIGLGMLYVPLDSVVKVF
SISYVLVCVSVVASMAIAGFFIGNLMKMYPIESAIVTCCHSGLGGTGDVAILSASNRMSL
MPFAQISTRLGGASTVIIASILLRIFA