Protein Info for AO353_24320 in Pseudomonas fluorescens FW300-N2E3

Annotation: MBL fold metallo-hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 294 PF00753: Lactamase_B" amino acids 19 to 200 (182 residues), 55.6 bits, see alignment E=3.5e-19

Best Hits

Swiss-Prot: 61% identical to Y2304_BURP1: Probable metallo-hydrolase BURPS1710b_2304 (BURPS1710b_2304) from Burkholderia pseudomallei (strain 1710b)

KEGG orthology group: None (inferred from 65% identity to psp:PSPPH_0083)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VZA1 at UniProt or InterPro

Protein Sequence (294 amino acids)

>AO353_24320 MBL fold metallo-hydrolase (Pseudomonas fluorescens FW300-N2E3)
MYFGEKILVEEFFDEATSTFSYLLLDRSTMQCALIDSVLDYDPKSGRTKTESADRIVARA
TELGASVEWILETHLHADHLTAAQYLKQRLGGKIGIGNRIKDVQKVFSHLFHTEDELRSG
SDQFDILFDDNDTFHIGSLTAQALHTPGHTPACMTYLIQDEQAGLAFVGDTLFMPDYGTA
RCDFPGGDARTLFRSIHKILALPESTHIFMCHDYRPNGRPLAFITSVKAQKRENIHVREG
TSEDTFVNMRETRDATLDMPMLILPSVQINMRGGNMPEPETNGIRYLKIPLNTL