Protein Info for AO353_24285 in Pseudomonas fluorescens FW300-N2E3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 transmembrane" amino acids 17 to 39 (23 residues), see Phobius details amino acids 51 to 67 (17 residues), see Phobius details amino acids 77 to 96 (20 residues), see Phobius details amino acids 102 to 121 (20 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 153 to 173 (21 residues), see Phobius details amino acids 185 to 204 (20 residues), see Phobius details amino acids 210 to 232 (23 residues), see Phobius details amino acids 243 to 262 (20 residues), see Phobius details amino acids 268 to 286 (19 residues), see Phobius details PF00892: EamA" amino acids 16 to 145 (130 residues), 27.5 bits, see alignment E=1.5e-10 amino acids 160 to 285 (126 residues), 55.4 bits, see alignment E=3.7e-19

Best Hits

Swiss-Prot: 56% identical to Y1977_PSEAE: Uncharacterized protein PA1977 (PA1977) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 80% identity to pst:PSPTO_4266)

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WL35 at UniProt or InterPro

Protein Sequence (295 amino acids)

>AO353_24285 hypothetical protein (Pseudomonas fluorescens FW300-N2E3)
VSQHILDIPLFKGAKKVVLLTTLAMLAFAANSVLCRLALRHTEIDPTSFTLIRLVSGAIM
LWVLLAIKNTTPRPSGTWFGAFTLFTYAFAFSYAYLNLETGTGALLLFGAVQLTMLGYGY
CKGERMQGVAIVGLLLAIGGLVALLLPGTSAPALGSAGIMLLSGIAWAGYSLIGKQSNDP
LASTAGNFLRAIPMMLLASIPFVQDFSIDLLGAFCAIASGTIASGVGYAIWYAAIRSLSS
FRAATIQLSVPVLASLAGVLLLGEQLTVRLALTSLAVLGGIGLVLSDKRLANGAS