Protein Info for AO353_23975 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1006 transmembrane" amino acids 25 to 47 (23 residues), see Phobius details amino acids 280 to 300 (21 residues), see Phobius details PF13185: GAF_2" amino acids 394 to 538 (145 residues), 58.1 bits, see alignment E=2.4e-19 PF01590: GAF" amino acids 396 to 537 (142 residues), 50.9 bits, see alignment E=4.8e-17 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 549 to 710 (162 residues), 147.5 bits, see alignment E=1.5e-47 PF00990: GGDEF" amino acids 551 to 707 (157 residues), 168 bits, see alignment E=3.1e-53 PF00563: EAL" amino acids 730 to 962 (233 residues), 251.1 bits, see alignment E=2e-78

Best Hits

KEGG orthology group: None (inferred from 90% identity to pba:PSEBR_a2263)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VYK9 at UniProt or InterPro

Protein Sequence (1006 amino acids)

>AO353_23975 diguanylate cyclase (Pseudomonas fluorescens FW300-N2E3)
MEPLTLDVISRLGRQLLPQSLRAQFTLAFLTLALLILAGGATAVYALRTSNSATRQLTDE
RLVRMQNGQDMLHRTLLIERQTDQLLTTRSPDTLRSSYAATVEQLEALDQLVQQLTAANS
GVAILDMYQSSQMFRNTANIVAQLRESLLQTEVTFEQTLEDHTARLTAAQTRASLELAVL
LFDLPQAVDSDAVQRLQIRYEHLSRTTGKLPDADVEIPKLFSLRLKLINQHNVIQRFNEE
LKNEAGVLVALARAQSSAYTEDYREAVQRLVEASNRSQQWVLIMLGASLVFAWFVTRVFM
GRHVLVRLNAISRQLRQEHTDKTHLMMAEHGKDEIANMARAVNQFLKDRLQLEKKSVQLS
IATERLAVQNSRLEQEAIIRTGQGHVLELIARSTELAEVLDSLAHLVESQLEGMMVSILV
LDEDGKHLLHGAAPSLPKAYNQLIDGIAIGPNVGSCGTAVYRREPVIVTNIELDPLWEDY
RSIAAPYGFRACWSTPILSHERKVLGTFALYSNTVRSPGSTETRLIDMATPLAGIAIERQ
LTEQRIRFMGDHDALTGLPNRTLLEDRLKQAILYAQRYNRQVTVAFLDLDKFKLVNDSLG
HSAGDELLKTVAQRMLECVRRTDTVVRLGGDEFVIILFDQPSDLDGVTPALHKIQEAILR
PIQLSGHTLHVTCSMGLATYPADGSDTDTLLSNADAAMYRAKELGRNSYQFYTSEMNNKV
QGKLAMQDGLRNALNHDEFLLLYQPQVDLQSGQIIGVEALIRWQHPELGMVSPIKFIPQA
EETGLIVPIGDWVIHTACRQNKDWQDAGWPPITMSVNISARQFIERDLIDRVRHALKETG
LDPMYLELELTESLIMQDLQQAINKMKELQSMGISLSIDDFGTGYSSLAALKSFPIARLK
IDQSFVRDLPDNENDKAIATAVISLGHTLNLRVIAEGVETQEQQTFLHENGCDEIQGHFF
SRAVSAEEISLLLRTPRLPQSSRIASPDSARRKRDVKRPSSPMPGH