Protein Info for AO353_23915 in Pseudomonas fluorescens FW300-N2E3

Annotation: chemotaxis protein CheY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1155 transmembrane" amino acids 20 to 44 (25 residues), see Phobius details amino acids 184 to 206 (23 residues), see Phobius details PF05227: CHASE3" amino acids 46 to 178 (133 residues), 130.8 bits, see alignment E=1e-41 PF13185: GAF_2" amino acids 252 to 388 (137 residues), 66.9 bits, see alignment E=6.7e-22 PF01590: GAF" amino acids 255 to 387 (133 residues), 40.6 bits, see alignment E=1.1e-13 PF00512: HisKA" amino acids 487 to 554 (68 residues), 61.1 bits, see alignment 2.5e-20 PF02518: HATPase_c" amino acids 602 to 712 (111 residues), 103.3 bits, see alignment E=3.1e-33 PF00072: Response_reg" amino acids 766 to 871 (106 residues), 56.2 bits, see alignment E=1.1e-18 amino acids 887 to 995 (109 residues), 52.9 bits, see alignment E=1.2e-17 amino acids 1033 to 1145 (113 residues), 93.1 bits, see alignment E=4e-30

Best Hits

KEGG orthology group: K00936, [EC: 2.7.3.-] (inferred from 83% identity to pfo:Pfl01_2646)

Predicted SEED Role

"Signal transduction histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WKX1 at UniProt or InterPro

Protein Sequence (1155 amino acids)

>AO353_23915 chemotaxis protein CheY (Pseudomonas fluorescens FW300-N2E3)
MTSSSSVDEQGFRKLLSRNVSLPLGMGVLSAVFFVSLITYLLSVIQWLEHTDRVINNANE
ALTLTVDLETGMRGFLLSGDEHFLDPYETAKPRIIAALKTLQELTADNPPQVDRLRRLEA
LQVEWNDYAQTMIDLQHRSGDYRAAVKSGRGKRLTDEIRKQYEDVIDMEQQLRITRNEGV
RRTTIWSISLYLLLVAGISGLLAYVGRRDLLSLSQKYSANLAAQQSSAQRLEQQAWLRNG
QTELAEQVLGQLTLNRLGRNILQFCAQYLGSAVGAIYAKEEHGGLTRIASYGFSREQEGR
EQAINSGEGITGQVAQQGRLIRLDEVPAGYFKVSSGLGEGVPCSVLVVPTRDDDRVNGVI
ELGFLHALTERDVELLELIAGNIGTSIEAARYRQRLQEVLAQTQQLNEELQVQQEELKTA
NEELEEQSRVLKESQWHLETQQAELEQTNEQLAEQRDAMDRKNGELNQAQVQLEERAQEL
QRSSQYKSEFLANMSHELRTPLNSSLILARLLAENPQQNLSAEQVTFAESIYSAGNDLLN
LINDILDISKVEAGKLVIRPENTSVARLVDGLRGMFEPLAADKHLAFNLELQTDAPLMVF
TDRQRLEQVLKNLLSNAIKFTEKGSVSLTVARQAGEGIAFIVRDSGIGIASDQQESIFEA
FRQADGSTNRRYGGTGLGLSISRDLAALLGGSISVISEPGQGSSFTLVLPVQYVDLGDEP
RELLKIVPVVAAPKVTAAPAPLIVEAKIPRFADDRDKAPFTRRCMLVVEDEVNFAHILFD
LAHELGYQCLVAHGADEGYDLAKRFMPDAILLDMRLPDHSGLTVLQRLKEHAETRHIPVH
VISVEDRVEAAMHMGAIGYALKPTTREELKDVFARLEAKLTQKVKRVLLVEDDDLQRDSI
ARLIGDDDIEITAVGLAQEALDLLRETIYDCMIIDLKLPDMLGNDLLKRMSTEDICSFPP
VIVYTGRNLTRDEEADLRKYSRSIIIKGARSPERLLDEVTLFLHKVESRLSHERQTMLKT
ARSRDRVFEGRKVLLVDDDVRNIFSLTSALEHKGAIVVIGRNGREAIEKLNEVDDIDLVL
MDVMMPEMDGFEATLEIRKDPRWRKLPIIAVTAKAMKDDQERCLQAGSNDYLAKPIDLDR
LFSLIRVWLPKMERI