Protein Info for AO353_23770 in Pseudomonas fluorescens FW300-N2E3

Annotation: malto-oligosyltrehalose trehalohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 transmembrane" amino acids 400 to 419 (20 residues), see Phobius details amino acids 514 to 530 (17 residues), see Phobius details amino acids 578 to 595 (18 residues), see Phobius details TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 21 to 552 (532 residues), 677.7 bits, see alignment E=4.9e-208 PF00128: Alpha-amylase" amino acids 130 to 214 (85 residues), 46.8 bits, see alignment E=3.2e-16 PF11941: DUF3459" amino acids 496 to 585 (90 residues), 56.5 bits, see alignment E=3.2e-19

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 79% identity to pba:PSEBR_a3007)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VTL4 at UniProt or InterPro

Protein Sequence (601 amino acids)

>AO353_23770 malto-oligosyltrehalose trehalohydrolase (Pseudomonas fluorescens FW300-N2E3)
MPLRTPETWPHGAVMLDAEHTRFALWAPDAFYVSVELEGGQSLPMLPQADGWFVIETRCP
AGTRYRYNIDGERDVPDPASRAQAGGLASPSVVVDPHAYRWQHLLWQGRPWHEAVIYELH
VGVLGGFAEVEKHLPRLAELGVTAIELMPLAQFPGERNWGYDGVLPYAPQSSYGTPDALK
HLIDSAHGLGLAVIIDVVYNHFGPDGNYLHHYAKGFFREDLHTPWGAAIDFRRREVRDFF
IDNALMWLLEYRFDGLRLDAVHAIEDPDFLQELAATVREHIDPPRHVWLTLENEHNQASL
LEQGFDAQWNDDGHNALHVLLTGETEAYYADFAERPSEKLARCLSQGFAYQGHTNRHGRA
RGEPSGHLPPSAFVLFLQNHDQIGNRAFGERLHQLTAPQALHAATVLMLLSPMIPLLFMG
DEYAADEPFLFFTSHHGVLARQVREGRRNEFAAFSAFADPHTREQIPDPNALHTFTASRP
TLTSSEPLTQQATLTLYRQLLLIRHREIIPRLPGAHALGATVLATGAISARWHMGDGSLL
CIDLNLTAEPVSHSSHAHSRLLFEYPQRSFEALQQGTLAPYCALVSLTAAAPLLPTTGER
P