Protein Info for AO353_23740 in Pseudomonas fluorescens FW300-N2E3

Annotation: autotransporter subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF03797: Autotransporter" amino acids 125 to 329 (205 residues), 87.5 bits, see alignment E=6.4e-29 TIGR01414: outer membrane autotransporter barrel domain" amino acids 128 to 353 (226 residues), 69.7 bits, see alignment E=1.2e-23

Best Hits

KEGG orthology group: None (inferred from 65% identity to pfo:Pfl01_2537)

Predicted SEED Role

"outer membrane autotransporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0S2 at UniProt or InterPro

Protein Sequence (353 amino acids)

>AO353_23740 autotransporter subunit beta (Pseudomonas fluorescens FW300-N2E3)
MNTSLIQHEIKITLYTVSTSLLLCTSMEVQASPTENEAMPWYQQDVPALALPTPDTSNSI
RFIRNPGLDNQLLTVEQAAPSAWDRVYGQPARSAEHDVLGSPFSGMSGSELKGPALITLQ
SSGGSTQRVGLMGGTNQFQGNGNGLVNSAIVDPNNTLNVQGPNLGAYWSLTGANGWHVDL
TASGGRVNGYSLNDQGQRQAAEGSAMTLSVQGGFPIGISENWVIEPQAELINQRISLDTP
YAGSGNASSTDLTSWSGRVGASLKGSYDIKGLPIEPYVRTNFWHTVNTSDTVTLGQVDKL
SSSRNASSIELGLGLVARVTPSVSLYVSGDYNSASVNNDLYGLIGSLGVRMRW