Protein Info for AO353_23295 in Pseudomonas fluorescens FW300-N2E3

Annotation: cystathionine beta-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01053: Cys_Met_Meta_PP
  • amino acids 15 to 389 (375 residues), 505.2 bits, E=4.5e-156

Best Hits

Swiss-Prot: 67% identical to METC_COXBU: Cystathionine beta-lyase (metC) from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)

KEGG orthology group: K01758, cystathionine gamma-lyase [EC: 4.4.1.1] (inferred from 88% identity to pfo:Pfl01_3249)

MetaCyc: 53% identical to cystathionine gamma-lyase (Helicobacter pylori 26695)
Cystathionine gamma-lyase. [EC: 4.4.1.1]

Predicted SEED Role

"Cystathionine gamma-lyase (EC 4.4.1.1)" in subsystem Cysteine Biosynthesis or Glycine and Serine Utilization or Methionine Biosynthesis or Methionine Degradation (EC 4.4.1.1)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• glycine betaine degradation III (7/7 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• D-serine degradation (3/3 steps found)
• L-cysteine degradation II (3/3 steps found)
• L-serine degradation (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
• L-cysteine biosynthesis III (from L-homocysteine) (2/2 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (4/5 steps found)
• glycine betaine degradation I (6/8 steps found)
• homocysteine and cysteine interconversion (3/4 steps found)
• L-methionine degradation II (2/3 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• L-threonine degradation I (4/6 steps found)
• hydrogen sulfide biosynthesis II (mammalian) (4/6 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• hypoglycin biosynthesis (4/14 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Nitrogen metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.4.1.1

Sequence Analysis Tools

See A0A0N9VYA4 at UniProt or InterPro

Protein Sequence (392 amino acids)

>AO353_23295 cystathionine beta-lyase (Pseudomonas fluorescens FW300-N2E3)
MSQHDENATPRAFATRVIHAGQTPDPTTGALMPPIYANSTYLQQSPGVHKGFDYGRSHNP
TRFALERCVADLEGGTQAFAFASGLAAISTVLELLDTGSHIVSGNDLYGGTFRLFDKVRR
RSAGHRFSFVDLTDLTAFEAALQDDTRMVWVETPSNPLLRITDLAAVARTCRERGIICVA
DNTFASPRIQRPLELGFDIVLHSTTKYLNGHSDVIGGIAVVGQNAELRERLGFLQNAVGA
IAGPFDAFLTLRGVKTLALRMERHCSNALELARWLSHQPQVARVYYPGLPSHPQHELAQR
QMHGFGGMISLDLDTDLAGAKRFLESVQIFALAESLGGVESLIEHPAIMTHASIPAETRA
ELGIGDALIRLSVGIEDVEDLRADLAQALAQI