Protein Info for AO353_22900 in Pseudomonas fluorescens FW300-N2E3

Annotation: acriflavine resistance protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1035 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 336 to 353 (18 residues), see Phobius details amino acids 360 to 381 (22 residues), see Phobius details amino acids 387 to 410 (24 residues), see Phobius details amino acids 431 to 455 (25 residues), see Phobius details amino acids 464 to 486 (23 residues), see Phobius details amino acids 529 to 548 (20 residues), see Phobius details amino acids 857 to 875 (19 residues), see Phobius details amino acids 882 to 902 (21 residues), see Phobius details amino acids 908 to 933 (26 residues), see Phobius details amino acids 954 to 974 (21 residues), see Phobius details amino acids 986 to 1012 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 7 to 1012 (1006 residues), 1064.3 bits, see alignment E=0 PF03176: MMPL" amino acids 241 to 487 (247 residues), 29.3 bits, see alignment E=6.4e-11

Best Hits

Swiss-Prot: 56% identical to MDTC_CITK8: Multidrug resistance protein MdtC (mdtC) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K07789, RND superfamily, multidrug transport protein MdtC (inferred from 69% identity to avn:Avin_28790)

Predicted SEED Role

"Acriflavin resistance plasma membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WGJ2 at UniProt or InterPro

Protein Sequence (1035 amino acids)

>AO353_22900 acriflavine resistance protein B (Pseudomonas fluorescens FW300-N2E3)
MNLSGPFIRRPVATMLLSLAIMLLGGVSFGLLPVSPLPQMDFPVIVVSASLPGASPEVMA
STVATPLERSFGAIAGVNTMSSRSSQGSTRVILQFDMDRDINGAAREVQAAINASRNLLP
SGMRSMPTYKKVNPSQAPIMVLSLTSDVLEKGQLYDLASTILSQSLSQVPGVGEVQIGGS
SLPAVRIELEPQLLNQYGVALDDVRNTIANANVRRPKGSIEDDQRMWQVQANDQLEKAKD
YEPLIIRYQNGSALRLGDVAKVRDSVEDRYNSGFFNDDAAVLLVINRQAGANIIKTVNDI
KAQLPALQAVLPASVKLNLAMDRSPVIKATLHEAEMTLLIAVALVILVVYLFLGNFRASL
IPTLAVPVSLVGTFAVMYLYGFSLNNLSLMALILATGLVVDDAIVVLENISRHIDEGIAP
MKAAYLGAKEVGFTLLSMNVSLVAVFLSILFMGGIIESLFREFSITLAASIVVSLVVSLT
LTPMLCARWLKPHTPGEENRLQRASRRANDWMVKHYSSGLDWVLRHKRLTLLSLFVTIGV
NIALYVVVPKTFLPQQDTGQLIGFVRGDNGLSFNVMQPKMETFRRAVLKDAAVESVAGFI
GGTNGTNNAFMLVRLKPIKERNISAQKVIERLRKEMPKVAGAQLMLMADQDLQFGGGREQ
TTSQYTYILQSGDLSALREWYPKVVTALKALPELTAIDAREGRGAAQVTLVVDRDQAKRL
GVDMSMVTAVLNNAYSQRQISTIYDSLNQYQVVMEVNPKYAQDPITLNQVQVITAAGARI
PLSAIAHYENSLENDRVSHEGQFASESISFDMAEGVTVEQGSAAIERAIAKLGLPEEVIA
KMAGTADAFAATQKSQPWMILGALVAVYLVLGVLYESYVHPLTILSTLPSAGVGALLSIY
VLGGEFSLISLLGLFLLIGVVKKNAILMIDLALQLERHQGMEPLESIRSACLLRLRPILM
TTLAAILGALPLLLSTAEGAEMRQPLGLTIIGGLIFSQVLTLFTTPVVYLYLDRLRHRVN
RWRGVRTDASLETPL