Protein Info for AO353_22865 in Pseudomonas fluorescens FW300-N2E3

Annotation: sulfite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03460: NIR_SIR_ferr
  • amino acids 49 to 110 (62 residues), 71.4 bits, E=4.1e-24
  • amino acids 346 to 394 (49 residues), 41.6 bits, 8.2e-15
• PF01077: NIR_SIR
  • amino acids 119 to 273 (155 residues), 149.3 bits, E=6.5e-48
  • amino acids 412 to 548 (137 residues), 54 bits, E=1.4e-18

Best Hits

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 92% identity to pfo:Pfl01_2513)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Sequence Analysis Tools

See A0A0N7H0Q2 at UniProt or InterPro

Protein Sequence (557 amino acids)

>AO353_22865 sulfite reductase (Pseudomonas fluorescens FW300-N2E3)
MYQYDDYDRALVFERVAQFRDQVERFMAGALSEEEFLPLRLQNGLYMQKHAYMLRVAIPY
GTLSARQMRTLASIARDYDRGYGHFTTRQNMQFNWIELAQVPDILERLAQVEMHAIQTSG
NCVRNITTEAFAGVAADELIDPRPLAEILRQWSTINPEFLFLPRKFKIAICSAEQDRAAI
MMHDIGLYLYRDAAGQMLLRVIVGGGLGRTPILGLQIREGLPWQHLLSYVEAVLRVYNRH
GRRDNKYKARIKILVKALGIEAFAQEVEQEWQHLKDGPAQLTDVEYQRVASAFVPPTYAP
LADTDLDFGTRLTENLAFARWVARNVQPHKVPGYTSVVLSTKPGMASPPGDVTDAQMDAV
ADWSEQFGFGEIRIAHEQNIVLPDVPKSDLYALWCLACEHGLGCANIGLLTDIIACPGGD
FCALANAKSIPIAQAIQARFDDLDYLHDLGDISLNISGCMNACGHHHIGNIGILGVDKNG
SEWYQITLGGSQGKNSALGKVIGPSFSAAEVPQVIERIIATFVRYRESEELFVDTLQRIG
LEPFKERVYPKSLEVVA