Protein Info for AO353_22740 in Pseudomonas fluorescens FW300-N2E3

Annotation: sterol desaturase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 52 to 70 (19 residues), see Phobius details amino acids 111 to 133 (23 residues), see Phobius details amino acids 139 to 158 (20 residues), see Phobius details amino acids 198 to 214 (17 residues), see Phobius details PF04116: FA_hydroxylase" amino acids 57 to 211 (155 residues), 52.8 bits, see alignment E=5.8e-18 PF10604: Polyketide_cyc2" amino acids 230 to 369 (140 residues), 63.2 bits, see alignment E=3.3e-21

Best Hits

KEGG orthology group: None (inferred from 77% identity to pfo:Pfl01_3129)

Predicted SEED Role

"Sterol desaturase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VWH7 at UniProt or InterPro

Protein Sequence (384 amino acids)

>AO353_22740 sterol desaturase (Pseudomonas fluorescens FW300-N2E3)
VRQTTQAFRARYRADIHRLYNPWLHGGFVLAFGVLGITLFWSTVHQVQPLEWLAVPLTLL
FFNFAVYVVHRHLGHHKKAFARLFYARHAGDHHSFFAPGYMTYDSARDWRVILFPAWLIV
LHSLAITLPAWWLLKHLNGNIAGLFAGCMILGYLAYEVFHACEHLPPGNPVTRLPWICQM
RRLHALHHRRELMQERNFNIVFPLMDYLFGTLYWEAEPDHSHLQTRRAPMTRLQHRIDIG
RDPISVLAYASTATRWPEWHPSSLKVDGPPGPLHAGARFEEDIHAGGRAGHLSWEVEEYL
PGRRWSARAQGDHGLELLLTYECASEGGSTRFVRTLEYRFSGRLMRLANRLLLKRRIEHE
SAASMLALRDMAQQTIPAQTGAIA