Protein Info for AO353_22715 in Pseudomonas fluorescens FW300-N2E3

Annotation: DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1025 TIGR00594: DNA polymerase III, alpha subunit" amino acids 6 to 970 (965 residues), 565.5 bits, see alignment E=1.3e-173 PF02811: PHP" amino acids 7 to 111 (105 residues), 65.5 bits, see alignment E=1.9e-21 PF07733: DNA_pol3_alpha" amino acids 269 to 524 (256 residues), 295.1 bits, see alignment E=1.3e-91 PF17657: DNA_pol3_finger" amino acids 527 to 693 (167 residues), 202.5 bits, see alignment E=7.2e-64 PF14579: HHH_6" amino acids 767 to 855 (89 residues), 75.3 bits, see alignment E=9e-25 PF01336: tRNA_anti-codon" amino acids 935 to 1004 (70 residues), 34.3 bits, see alignment 4.6e-12

Best Hits

Swiss-Prot: 85% identical to DNAE2_PSEPF: Error-prone DNA polymerase (dnaE2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 85% identity to pfo:Pfl01_3149)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WGG0 at UniProt or InterPro

Protein Sequence (1025 amino acids)

>AO353_22715 DNA polymerase (Pseudomonas fluorescens FW300-N2E3)
MSAGYAELHCLSNFSFQRGASSALELFERAKRQGYQALAITDECTLAGIVRAWQAAKALE
LPLIIGSEMRIENGPKLVLLVENLEGYQALCRLITHARRRTEKGQYRVLREDFNGPLPGL
LALWVPDAVDALEHGAWLKGIFGERLWLAVELHYGQNDARRLADWQALATRLQIPVVASG
DVHMHARGRRALQDTMTAIRLHVRVADAGQRLHPNGERHLRSLEALRELYPASLLEETLI
IARRCTFDLGQLRYQYPRELVPEGQTPTSWLRRLTEQGLSRRWPEGPDSTVMALIEKELK
LIAELGYESYFLTVEDIVSFARSQRILCQGRGSAANSAVCYALGITEIDPSRTSMLFERF
LSKERNEPPDIDVDFEHERREEVLQYVFQRYGRTRAALTAVVSTYHGAGAIRDVAKALGL
PPDQVNALADCCGHWSDDVPPLERLREGGFDPDSPVLRQVLSLTRQLIGFPRHLSQHPGG
FVISEQPLDSLVPVENAAMAERTIIQWDKDDLDMVGLLKVDILALGMLSAIRRCFDLLRD
HRGRDLSLANIPSEDPQTYEMISRADTIGVFQIESRAQMSMLPRLKPQNFYDLVIEVAIV
RPGPIQGGMVHPYLRRRNKEEPETYPSVQLEAVLKRTLGVPLFQEQVMQIAIVAADYSPG
EADQLRRCMAAWKRHGGLEPHQKRLREGMLKNGYSEEFAAQIFEQIKGFGSYGFPESHAA
SFALLTYASCWLKCHEPAAFACALINSWPMGFYSPDQILQDARRHHLQIRPVDVAASDWD
CSLEPIDGEQPAIRLGLRMIKGFREDDARRIEAARLQGEFIDIADLGERARLDVRAQEQL
ADAGALRRLAGDRHRARWEVAGVQKQLGLFAGLPSQEEPCVVLPKPTVSEDLQADYSSVG
TTLGPHPLALLRDELRARRCRSSRELLEVEHGRNVSVAGLVTGRQRPGTASGVTFVTLED
EFGNINVVVWRDLAERQRKALIGSQLLKIDGRWEKVGEVRHLIAGRLSDLSPLLNGISVH
SRDFH