Protein Info for AO353_22470 in Pseudomonas fluorescens FW300-N2E3
Annotation: MFS transporter
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 62% identical to PHAG_PSEAE: (R)-3-hydroxydecanoyl-ACP:CoA transacylase (phaG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: None (inferred from 69% identity to pfl:PFL_1429)MetaCyc: 44% identical to 3-(3-hydroxyalkanoyloxy)alkanoate synthase (Pseudomonas aeruginosa PA14)
RXN-11762
Predicted SEED Role
"(R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-)" in subsystem Rhamnolipids in Pseudomonas (EC 2.4.1.-)
MetaCyc Pathways
- rhamnolipid biosynthesis (1/3 steps found)
KEGG Metabolic Maps
- Anthocyanin biosynthesis
- Carotenoid biosynthesis - General
- Flavone and flavonol biosynthesis
- Flavonoid biosynthesis
- Fructose and mannose metabolism
- Glycerolipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- High-mannose type N-glycan biosynthesis
- Keratan sulfate biosynthesis
- Lipopolysaccharide biosynthesis
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Peptidoglycan biosynthesis
- Sphingolipid metabolism
- Zeatin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.-
Use Curated BLAST to search for 2.4.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WGC6 at UniProt or InterPro
Protein Sequence (294 amino acids)
>AO353_22470 MFS transporter (Pseudomonas fluorescens FW300-N2E3) MMPKTAIVEICKKYKVYTEHYLNAAADKTIILVNGSLATTASFAQTVRYLQPRFNVVLYD QPYAGQSREHNLHGQPIRKEDEAQILLELIEHFSVHHVLSFSWGGAATLLALAQRPRRIE KAVINSFAARINTPMRDYLESGLNFLQACDRPNVGHLINSTIGKHLPSLFKRFNHRHVSS LAEHEYRQMHFHVNEILTQDIHCYSVCADAIEVPMLFVNGEWDEYTSVEDARLFAAHARQ SRFHTIKNAGHFLDMEHPAAWHDTRQALLGFLQPACGAPSQVRHHAYSREASLM