Protein Info for AO353_22290 in Pseudomonas fluorescens FW300-N2E3

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF20434: BD-FAE" amino acids 89 to 198 (110 residues), 63.1 bits, see alignment E=2.8e-21 PF07859: Abhydrolase_3" amino acids 104 to 310 (207 residues), 243.5 bits, see alignment E=2.1e-76

Best Hits

Swiss-Prot: 37% identical to YR526_MIMIV: Putative alpha/beta hydrolase R526 (MIMI_R526) from Acanthamoeba polyphaga mimivirus

KEGG orthology group: None (inferred from 96% identity to pfo:Pfl01_1969)

Predicted SEED Role

"Esterase/lipase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WK54 at UniProt or InterPro

Protein Sequence (338 amino acids)

>AO353_22290 alpha/beta hydrolase (Pseudomonas fluorescens FW300-N2E3)
MNIKQTLSASLLALSIGNAFAAGSPGVEHNTQAFLDALAAGGGKPLEQLSPKDARAVLTG
AQASVKVDLSGVEVSDKAIKVDGQTINLKVVRPAGAKGELPVFMFFHGGGWVLGDFPTHQ
RLIRDLVVGSGAVAVYVDYTPSPEAHYPTAINQAYAATKWVAEHGEDIGVDGKRLAVAGN
SVGGNMAAVVALMAKEQNTPALRFQLLMWPVTNAGFDNGSYQQFAEGHFLTKGMMQWFWD
NYTTSPAERAQIHASPLNASAEQLKGLPAALVQTAEFDVLRDEGEGYARHLDAAGVPVTS
VRYNGMIHDFGLLNPLSQIPEVKAAVRQAAAELKTHLN