Protein Info for AO353_22085 in Pseudomonas fluorescens FW300-N2E3

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 47 to 65 (19 residues), see Phobius details amino acids 76 to 93 (18 residues), see Phobius details amino acids 98 to 123 (26 residues), see Phobius details amino acids 133 to 157 (25 residues), see Phobius details amino acids 163 to 182 (20 residues), see Phobius details amino acids 212 to 231 (20 residues), see Phobius details amino acids 242 to 263 (22 residues), see Phobius details amino acids 275 to 293 (19 residues), see Phobius details amino acids 313 to 335 (23 residues), see Phobius details amino acids 348 to 372 (25 residues), see Phobius details amino acids 378 to 399 (22 residues), see Phobius details PF07690: MFS_1" amino acids 17 to 294 (278 residues), 130.7 bits, see alignment E=3.3e-42

Best Hits

Swiss-Prot: 41% identical to NARK1_THETH: Probable nitrate/nitrite antiporter NarK1 (narK1) from Thermus thermophilus

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 89% identity to pba:PSEBR_a3150)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VYD4 at UniProt or InterPro

Protein Sequence (434 amino acids)

>AO353_22085 MFS transporter (Pseudomonas fluorescens FW300-N2E3)
VIQPRVRQGLVLGMSTLAFTVCFMVWMMFAVLGVPIKELLQLNETQFGLLAATPVLTGSL
VRLPLGLLTDRFGGRSVFFLLMLSCVAPLYLISHATAYWQFLVLGLFVGLAGGSFSVGIA
YVAKWFDKENQGFAMGIFGAGNAGAAVTKFLAPALIAAGSWQLVPKVFSAILFITALLFW
FVSAENKNHRSAAGASLREQLRSLKDPAVWRYCQYYSIVFGGYVALALWMTKYYVQEYGF
SLQSAALLAACFSLPGGVLRAVGGWMSDRWGAQSVTWWVLWVSWICLFLLSYPQTQLQVQ
TLNGPMDFHIGLNPALFTVLLFVMGIAFAFGKASVFKYIANDYPKNMGAVSGIVGLAGGL
GGFVLPILFGALVDLTGVRSSCFMLMYGVVWVSLTWMYFSEIRKTPVFGKELPVTTSPFS
SIAQGEEHVRSAKA