Protein Info for AO353_21975 in Pseudomonas fluorescens FW300-N2E3

Annotation: AAA family ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 PF00158: Sigma54_activat" amino acids 24 to 136 (113 residues), 22.1 bits, see alignment E=2.2e-08 PF07728: AAA_5" amino acids 34 to 168 (135 residues), 125 bits, see alignment E=4.5e-40 PF08406: CbbQ_C" amino acids 181 to 264 (84 residues), 98.7 bits, see alignment E=3.6e-32

Best Hits

Swiss-Prot: 91% identical to NIRQ_PSEST: Denitrification regulatory protein NirQ (nirQ) from Pseudomonas stutzeri

KEGG orthology group: K04748, nitric oxide reductase NorQ protein (inferred from 90% identity to pba:PSEBR_a3171)

Predicted SEED Role

"Nitric oxide reductase activation protein NorQ" in subsystem Denitrification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WNT5 at UniProt or InterPro

Protein Sequence (267 amino acids)

>AO353_21975 AAA family ATPase (Pseudomonas fluorescens FW300-N2E3)
MDRIPSCEHHVEPFYQPLNNEQALFEQAWRHGMPVLIKGPTGCGKTRFVQHMAHRLKLPL
YTVACHDDLSAADLIGRHLIGAQGTWWQDGPLTRAVREGGICYLDEVVEARQDTVVVLHP
LADDRRELFLERIGEVLKAPPSFMLVVSYNPGYQNLLKGMKPSTRQRFVAMRFGYPPVAD
EERIVAREAQVDCALAAQVVRLGQALRRLDQHDLEEVASTRLLIFTARMIRSGMSPREAC
MACLAEPLSDDPLTVAALMDVVDVHFG