Protein Info for AO353_21915 in Pseudomonas fluorescens FW300-N2E3
Annotation: coproporphyrinogen III oxidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 49% identical to HEMN_PSEAE: Oxygen-independent coproporphyrinogen III oxidase (hemN) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 82% identity to pba:PSEBR_a2640)MetaCyc: 49% identical to coproporphyrinogen III dehydrogenase (Escherichia coli K-12 substr. MG1655)
HEMN-RXN [EC: 1.3.98.3]
Predicted SEED Role
"Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.99.22)
MetaCyc Pathways
- heme b biosynthesis II (oxygen-independent) (3/4 steps found)
- superpathway of heme b biosynthesis from uroporphyrinogen-III (4/6 steps found)
- 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) (3/9 steps found)
- superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.22
Use Curated BLAST to search for 1.3.98.3 or 1.3.99.22
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WNS4 at UniProt or InterPro
Protein Sequence (462 amino acids)
>AO353_21915 coproporphyrinogen III oxidase (Pseudomonas fluorescens FW300-N2E3) MTISFDFNRALVEKYDRPGPRYTSYPTAPQFHQAFAVDDYQRAASDSNQAATPKSLSVYI HIPFCKSLCYYCACNKIITRKTHRAEEYLTYLKREIVMQAALFDRTRKLTQLHLGGGTPT YLTSEQLAELMDCLHQAFDMDDSDDHEFSIEVDPRTISTEQIQSLRRLGFNRLSFGVQDF DPDVQAAVNRQQSEGQIYALVAAARQAQFKSISVDLIYGLPLQTVNSFDVTLGKIIALRP DRIAAYSYAHLPELVRAQRLIRPADMPPPERKLELLELTIRRLTEAGYVYIGMDHFALPD DELALARANGTLQRNFQGYSTHADCDLIGLGVSSIGKVGDSYSQSVKELSQYYARLDQGL LPVHRGYRLSADDQLRREVISDLMCHGRIDFGKFETRHAICFTEYFADALMQLDEHVRDG LLEIHEDALVLLPHGHLMMRSAAMAFDAYLDGDQKGRFSRTV