Protein Info for AO353_21875 in Pseudomonas fluorescens FW300-N2E3

Annotation: proline iminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 TIGR01250: proline-specific peptidase" amino acids 29 to 290 (262 residues), 263.2 bits, see alignment E=1.7e-82 PF00561: Abhydrolase_1" amino acids 32 to 279 (248 residues), 84.9 bits, see alignment E=1.1e-27 PF12146: Hydrolase_4" amino acids 51 to 278 (228 residues), 49.4 bits, see alignment E=5.7e-17 PF12697: Abhydrolase_6" amino acids 52 to 283 (232 residues), 62.6 bits, see alignment E=1.4e-20

Best Hits

Swiss-Prot: 39% identical to PIP_BREBN: Proline iminopeptidase (pip) from Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 88% identity to pba:PSEBR_a717)

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WJZ1 at UniProt or InterPro

Protein Sequence (295 amino acids)

>AO353_21875 proline iminopeptidase (Pseudomonas fluorescens FW300-N2E3)
MWREIEPDQRYTVEVDGHNLAVYGFGDGHEVLFCLNGGPGLPCDYLRDAHGWLKDKGLRV
IAFDQLGTGASAKPVDKSLWDITRYVAEVETVREALDLGVIHLLGHSWGGWLAIEYAIHH
PQSVKTLILENTAGDIPHLSQELERLRGALGSETVAMMQRHEALGTLNHPQYQAAITLLN
YRHVCRLDEWPAPLARSLSNWNMGPYVTMQGPNEFLYTGNLKNWNRIEQMVDFDMPVLIT
TGQHDEQTPACAMRMKMALKDAELHVFPNSSHMPFYEEPQAYFPVLLDFLQRHRS