Protein Info for AO353_21850 in Pseudomonas fluorescens FW300-N2E3
Annotation: diguanylate cyclase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02035, peptide/nickel transport system substrate-binding protein (inferred from 94% identity to pba:PSEBR_a712)Predicted SEED Role
"Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2); Putative hemin-binding lipoprotein" (TC 3.A.1.5.2)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VYA1 at UniProt or InterPro
Protein Sequence (504 amino acids)
>AO353_21850 diguanylate cyclase (Pseudomonas fluorescens FW300-N2E3) MKSKHLKLLTAATLAACTLAAGVSQAAGVLTIGCREESTTFDPIKSAQNRDNWVFSNVYD VLVRVDNAGTKLVPGLAESWKISDDGLIYTFKLRSAKFSDGSPITASDAVFSLLRIRDNK GSLWSDSYKIIDKAEAIDPQTLVVTLKTPSVPFLSQLALPNVSILSQKAMEKMGEEAYAQ EPVASGAFTVKEWLRGDRVVLEKNPNFWQADKVSLDGVEWISIPDDNTRMLKVQAGELDS AIFVPFSRVETLKKDSNLVVHMNPSTREDHLLINHEHGLLAKPEVRQALDLAINKKSLVD TVTFGHGQVAYSYIPKGALYHNADNLQRPYNPEKAKKMLADAGASRLKLNYVVNAGNEAD EQIAVLVQQQLAAAGVTVTLQKVDPTQSWQMLIDGDYDLSVMYWTNDILDPDQKTTFVLG HDTNMNYMTRYKNDKVKDLVATARVEVDPSKRKQMYTDLQTMAKQDVNWIDLYYSPYINV SRKNIEHFQQNPLGRFSLEETVKN