Protein Info for AO353_21365 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Inositol 2-dehydrogenase (EC 1.1.1.18)
Rationale: Specifically important for utilizing m-Inositol. Automated validation from mutant phenotype: the predicted function (1.1.1.18) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: inositol 2-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01408: GFO_IDH_MocA
  • amino acids 4 to 125 (122 residues), 91 bits, E=9.1e-30
• PF02894: GFO_IDH_MocA_C
  • amino acids 137 to 325 (189 residues), 98.4 bits, E=5.5e-32

Best Hits

Swiss-Prot: 93% identical to IOLG_PSEFS: Inositol 2-dehydrogenase (iolG) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00010, myo-inositol 2-dehydrogenase [EC: 1.1.1.18] (inferred from 96% identity to pba:PSEBR_a3350)

MetaCyc: 73% identical to myo-inositol 2-dehydrogenase (Klebsiella aerogenes)
Inositol 2-dehydrogenase. [EC: 1.1.1.18]

Predicted SEED Role

"Myo-inositol 2-dehydrogenase (EC 1.1.1.18)" in subsystem Inositol catabolism (EC 1.1.1.18)

MetaCyc Pathways

• myo-inositol degradation I (7/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (7/10 steps found)
• myo-inositol degradation II (1/5 steps found)
• streptomycin biosynthesis (2/18 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.18

Use Curated BLAST to search for 1.1.1.18

Sequence Analysis Tools

See A0A0N9WLK0 at UniProt or InterPro

Protein Sequence (336 amino acids)

>AO353_21365 Inositol 2-dehydrogenase (EC 1.1.1.18) (Pseudomonas fluorescens FW300-N2E3)
MSLKLGVIGTGAIGQDHIRRCSQTLLNSQVVAVTDINLQQAAKVVADLKLTAEVYPDGHA
LIKAPDVEAILVTSWGPSHEEFVLAAIAAGKPVFCEKPLAVTAEGCRKIVEAEVAHGKRL
VQVGFMRPYDEGYRALKAVIDSGQIGEPLMLHCAHRNPTVGENYKTDMAITDTLIHELDV
LRWLLDDDYVSVQVVFPRKTSKAHAHLKDPQIVLLETAKGTRIDVEVFVNCQYGYDIQCE
VVGETGIAKLPEPSQVQMRSGAKLSNAILMDWKDRFIAAYDVELQAFIDGVRAGQVGGPS
AWDGFAAAVAADACIEAQQSGAIVNVALPDRPRFYG