Protein Info for AO353_21360 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22)
Rationale: Specifically important for utilizing m-Inositol.
Original annotation: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione acylhydrolase (decyclizing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 643 TIGR04377: 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase" amino acids 5 to 641 (637 residues), 968.4 bits, see alignment E=8.6e-296 PF02776: TPP_enzyme_N" amino acids 7 to 134 (128 residues), 61.3 bits, see alignment E=1.2e-20 PF00205: TPP_enzyme_M" amino acids 221 to 353 (133 residues), 114.5 bits, see alignment E=4.8e-37 PF02775: TPP_enzyme_C" amino acids 439 to 598 (160 residues), 118.5 bits, see alignment E=3.5e-38

Best Hits

Swiss-Prot: 66% identical to IOLD_GEOKA: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD) from Geobacillus kaustophilus (strain HTA426)

KEGG orthology group: K03336, 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase [EC: 3.7.1.-] (inferred from 92% identity to pba:PSEBR_a3351)

MetaCyc: 57% identical to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (Bacillus subtilis subtilis 168)
RXN-14149 [EC: 3.7.1.22]

Predicted SEED Role

"Epi-inositol hydrolase (EC 3.7.1.-)" in subsystem Inositol catabolism (EC 3.7.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.- or 3.7.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WMG8 at UniProt or InterPro

Protein Sequence (643 amino acids)

>AO353_21360 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (Pseudomonas fluorescens FW300-N2E3)
MSTTRLTMAQALVKFLDNQYVEVDGVQSKFVAGIFTIFGHGNVLGLGQALEQDSGDLIVH
QGRNEQGMAHAAIGFAKQNLRRKIYACSSSVGPGAANMLTAAATATANRIPLLLLPGDVY
ASRQPDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPEQLMSAAIHAMRVLTDPAETG
AVTLALPQDVQAEAYDYPDYFLQKRVHRIDRRPATEAMLGDAVALLKGKRKPLIICGGGV
KYSGANTALQAFAERFDIPFAETQAGKSAVVSSHPLNVGGIGETGCLAANLLAKEADLII
GIGTRYSDFTTSSKWLFQHPEVKFLNLNISPCDALKLDGVQLLADARSGLQALSEALTDY
RSSWGDQPHQAKAQLDEEVDRVYQVEYQTQDFIPEINDHMEPAVLREFIELTGSCLTQSR
VLGVLNQTLADDAVIVAAAGSLPGDLQRSWRSKGVNTYHVEYGYSCMGYEVNAALGVKLA
EPTREVYALVGDGSYMMLHSELATSIQERRKINVILLDNMTFGCINNLQMEHGMDSFGTE
FRFRNPSTCKLDGGFVPVDFAMSAAAYGCKTYKVNTVEQLQAALADARLQTVSTLIDIKV
LPKTMIHKYLSWWRVGVAQVSTSARTDAVAKTLNERLAKARQY